Anti-PKC gamma Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - P05129

Core Product Specifications and Parameters

Biological Background: PKC gamma Function and Localization

• Protein kinase C gamma type (PKCγ) is a serine/threonine kinase encoded by the PRKCG gene, belonging to the protein kinase C family. It is a calcium-activated, phospholipid- and diacylglycerol (DAG)-dependent enzyme.
• Plays pivotal roles in neuronal cells, including regulation of glutamate receptors (GRIA4 and GRIN1), modulation of synaptic plasticity, long-term potentiation (LTP), memory acquisition, and learning.
• Involved in opioid receptor signaling and pain pathways; contributes to morphine tolerance, neuropathic pain, and desensitization of mu-type opioid receptors.
• Mediates ethanol sensitivity and tolerance by modulating GABA(A) receptors, affecting behavioral responses to alcohol.
• Functions in cerebral ischemia: modulates neurotransmission and cell survival, and insulin-induced necrosis inhibition to minimize ischemic injury. Also activated by oxidative stress, leading to connexin-43 phosphorylation and gap junction disassembly.
• Phosphorylates p53/TP53, promoting p53-dependent apoptosis in response to DNA damage.
• Stabilizes the core circadian clock component BMAL1 by interfering with its ubiquitination, contributing to phase resetting of the cerebral cortex clock during restricted feeding.
• Phosphorylates and activates LRRK1, which then phosphorylates RAB proteins involved in intracellular trafficking. Related references: PMID:36040231
• Subcellular localization: cytoplasm, perinuclear region, cell membrane, synapse, synaptosome, and dendrites. Specifically expressed in Purkinje cells of the cerebellar cortex.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the C-terminal region (aa 558–697) of human PKC gamma, which may be suitable for detecting total protein levels in Western blot (WB). Validate the antibody in your specific sample system and optimize dilution.
• For immunofluorescence (IF/ICC), consider using appropriate fixation and permeabilization protocols. Confirm specificity with positive and negative control cell lines/tissues.
• In ELISA applications, test antibody pairing compatibility and optimize coating antigen concentrations for optimal signal-to-noise ratio.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio integrates this PKC gamma antibody into its comprehensive IVD raw-material portfolio, providing one-stop access to validated antibody pairs, antigens, and ancillary reagents for diagnostic kit development and neuroscience research. We support sourcing for kinase and signal transduction targets, offering bulk supply and custom solutions. Contact us to discuss your specific procurement needs.