Anti-PKC gamma Monoclonal Antibody for WB - P05129

Core Product Specifications and Parameters

Biological Background: PKC gamma Function and Localization

• Protein kinase C gamma type (PKC gamma, also known as PKCC, PKCG, SCA14, PKCgamma) is a calcium-activated, phospholipid- and diacylglycerol (DAG)-dependent serine/threonine kinase encoded by the PRKCG gene. Alternative splicing may produce multiple isoforms.
• Plays a central role in neuronal signaling by phosphorylating glutamate receptors such as GRIA4/GLUR4 and GRIN1/NMDAR1, thereby regulating synaptic plasticity, long-term potentiation (LTP), and memory processes.
• Modulates opioid receptor signaling in the spinal cord and limbic forebrain, contributing to morphine-induced reinforcing effects, desensitization of mu-type opioid receptors, and chronic neuropathic pain.
• Exhibits neuroprotective functions: during cerebral ischemia, it modulates neurotransmission and cell survival; in response to oxidative stress, it phosphorylates connexin-43 (GJA1) to disassemble gap junctions, and phosphorylates p53/TP53 to promote apoptosis upon DNA damage.
• Localizes to the cytoplasm, perinuclear region, cell membrane, and neuronal projections including synapses, synaptosomes, and dendrites.
• Highly expressed in Purkinje cells of the cerebellar cortex, and is required for the elimination of multiple climbing fibers during cerebellar development.
• Involved in circadian rhythm regulation by stabilizing the core clock component BMAL1 in the cerebral cortex, thereby suppressing its ubiquitination-mediated degradation (by similarity).
• Phosphorylates and activates LRRK1, which in turn phosphorylates RAB proteins involved in intracellular trafficking (PubMed:36040231). Related references: PMID:36040231

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 604-697, a C-terminal region of human PKC gamma. This design may enable recognition of full-length PKC gamma and certain isoforms; validation in the relevant sample system is advised.
• This antibody is validated for Western blot, where it detects a band near 78 kDa. For ELISA, use appropriate controls and consider optimizing the protocol for your specific assay conditions.
• Due to reported cross-reactivity with mouse and rat, this antibody can be applied in rodent models. However, verify reactivity and specificity in your tissue or cell type of interest.
• If adapting for other applications (e.g., IHC, IF, IP), consult the literature and perform pilot experiments as performance may vary.

CamelBio: Your One-Stop Sourcing Bridge

• CamelBio is a one-stop sourcing partner for IVD raw materials, offering validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents to diagnostic manufacturers and research institutions.
• Our anti-PKC gamma monoclonal antibody supports investigations into neuronal signaling, neurodegeneration, pain mechanisms, and circadian biology.
• From rare target antibody sourcing to technical consulting, CamelBio bridges the gap from early research to clinical application, streamlining your development pipeline.