Anti-PKC epsilon Rabbit Polyclonal Antibody for WB, IHC-P, ELISA - Q02156

Core Product Specifications and Parameters

Biological Background: PKC epsilon Function and Localization

• Protein kinase C epsilon type (PKC epsilon, also known as nPKC-epsilon) is a calcium-independent, phospholipid- and diacylglycerol (DAG)-dependent serine/threonine-protein kinase encoded by the PRKCE gene in humans. It belongs to the protein kinase C family and contains a zinc-finger phorbol-ester/DAG-type domain.
• PKC epsilon plays key roles in regulating cytoskeletal dynamics, including cell adhesion, motility, migration, and cell cycle progression. It mediates integrin-dependent signaling and phosphorylates substrates such as MARCKS and vimentin to control cell spreading and directional transport.
• This kinase is involved in neuron growth, synaptic plasticity, and ion channel regulation, contributing to neurite outgrowth and presynaptic facilitation. It also modulates pain signaling by phosphorylating TRPV1.
• PKC epsilon participates in immune responses by phosphorylating TICAM2/TRAM downstream of TLR4, leading to IRF3 activation and cytokine production. It also contributes to cancer cell invasion through STAT3 phosphorylation.
• In cardiac cells, PKC epsilon regulates myofilament function and excitation-contraction coupling, and its persistent phosphorylation of troponin I is implicated in dilated cardiomyopathy. It also plays a role in cardiomyocyte hypertrophy.
• PKC epsilon localizes to the cytoplasm, cytoskeleton, cell membrane, perinuclear region, and nucleus, enabling dynamic spatial regulation of its signaling activities.
• Additional functions include regulation of apoptosis in erythroid progenitors, modulation of insulin-induced AKT1 activation, and phosphorylation of NLRP5/MATER to modulate AKT pathway in cumulus cells. Related references: PMID:19542546
• PKC epsilon also phosphorylates and activates LRRK1, which in turn phosphorylates RAB proteins involved in intracellular trafficking. Related references: PMID:36040231
• At the protein level, PKC epsilon is expressed in cumulus cells, with broader expression likely in various tissues based on functional studies.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the C-terminal catalytic domain (aa 488-737), which is highly conserved among human, mouse, and rat PKC epsilon, supporting the reported cross-reactivity.
• For Western blot (WB), the expected band is around 84 kDa. Consider using whole-cell lysates from stimulated cells (e.g., PMA-treated) to enhance detection.
• In immunohistochemistry (IHC-P), appropriate antigen retrieval and permeabilization steps may be required due to the kinase's multi-compartment localization.
• ELISA performance may depend on the coating antigen and detection format; validate using recombinant protein or peptide standards.
• When studying PKC epsilon activation, consider assessing its phosphorylation status or translocation as activation markers, as the antibody may recognize both unphosphorylated and phosphorylated forms.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio empowers diagnostic manufacturers and researchers with a comprehensive portfolio of IVD raw materials, technical services, and consulting. For PKC epsilon-related signal transduction studies, we offer validated antibody pairs, bulk polyclonal antibodies, and ancillary reagents for ELISA and immunohistochemistry. Our global supply network enables efficient sourcing of rare targets and custom antibody development to accelerate your assay development from concept to clinic.