Anti-PKC-beta Rabbit Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - P05771

Core Product Specifications and Parameters

Biological Background: PKC-beta Function and Localization

• Protein kinase C beta (PKC-beta) is a calcium-activated, phospholipid- and diacylglycerol (DAG)-dependent serine/threonine-protein kinase that plays pivotal roles in B-cell receptor signaling, oxidative stress-induced apoptosis, and transcriptional regulation. Related references: PMID:11598012 PMID:20228790 PMID:25982116 PMID:19176525 PMID:25313067 PMID:36040231
• In B-cell activation, PKC-beta mediates canonical NF-kappa-B signaling by phosphorylating CARD11/CARMA1, promoting recruitment of BCL10-MALT1 and IKK complex activation. It also negatively regulates BTK through phosphorylation at Ser-180. Related references: PMID:11598012
• Under oxidative conditions, PKC-beta phosphorylates p66Shc at Ser-36, triggering mitochondrial reactive oxygen species production and apoptosis.
• As a coactivator of androgen receptor (AR)-dependent transcription, PKC-beta is recruited to AR target genes and specifically phosphorylates histone H3 at Thr-6 (H3T6ph), preventing demethylation of H3K4me. Related references: PMID:20228790
• In insulin signaling, PKC-beta may function downstream of IRS1 to regulate glucose transport, including phosphorylation of glucose transporters GLUT1 and GLUT4. Related references: PMID:25982116
• PKC-beta acts as an activator of ferroptosis by phosphorylating ACSL4 and promoting FOXK1-dependent LGALS13 secretion. Related references: PMID:35027735 PMID:40246981
• The protein localizes to the cytoplasm, nucleus, and membrane, and alternative splicing generates isoforms PKC-beta I and PKC-beta II.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 260-340 within the regulatory domain of human PKC-beta. For Western blotting, expect a major band at ~77 kDa. Validate sensitivity in your specific cell or tissue lysates.
• For immunohistochemistry (IHC-P), antigen retrieval conditions and antibody dilution may require optimization. Include appropriate positive and negative tissue controls.
• In immunofluorescence (IF/ICC), PKC-beta localizes to the cytoplasm, nucleus, and membrane; permeabilization is essential for nuclear detection.
• As a polyclonal antibody, lot-to-lot variability may occur; consider pre-adsorption against the immunogen for critical applications.
• Cross-reactivity with mouse and rat has been reported; confirm reactivity in your model system.

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