Anti-PKC alpha Rabbit PolymAb® for WB and ELISA - P17252

Core Product Specifications and Parameters

Biological Background: PKC alpha Function and Localization

• Protein kinase C alpha type (PKCα) is a calcium-activated, phospholipid- and diacylglycerol (DAG)-dependent serine/threonine-protein kinase encoded by the PRKCA gene. It belongs to the protein kinase C family and contains regulatory zinc-finger domains and a catalytic kinase domain.
• In cancer biology, PKCα is highly expressed in gliomas and breast cancers, where it acts as a tumor promoter and contributes to malignant phenotypes by regulating cell proliferation, apoptosis, and migration through pathways such as MAPK/ERK.
• PKCα plays critical cardiovascular roles: it negatively modulates myocardial contractility by phosphorylating cardiac troponin T (TNNT2), promotes cardiac hypertrophy via ERK1/2 signaling, and is required for cardiomyocyte hypertrophy upon PMA stimulation.
• In angiogenesis and platelet function, PKCα mediates VEGFA-induced endothelial cell proliferation and tube formation, and activates platelet integrin αIIbβ3 through the RAP1GAP pathway, facilitating calcium-induced platelet aggregation and thrombus formation.
• PKCα phosphorylates key downstream substrates: it phosphorylates CDC42-guanine exchange factor DOCK8 to enhance CDC42 activity during T cell migration, phosphorylates KLHL3 to decrease its interaction with WNK4 in response to angiotensin II, and activates LRRK1 involved in RAB-mediated intracellular trafficking. Related references: PMID:28028151 PMID:25313067 PMID:36040231
• Subcellularly, PKCα dynamically localizes to the cytoplasm, cell membrane, mitochondrion membrane, and nucleus, translocating between compartments in response to stimuli. Post-translational modifications include phosphorylation and acetylation.

Experimental Guidance and Technical Tips

• For Western blotting, consider using positive controls such as HeLa, MCF-7, or A431 cell lysates, which express endogenous PKCα. A starting dilution range should be empirically determined.
• Because the immunogen is recombinant human PKCα protein and cross-reactivity with mouse and rat is reported, validate the antibody in the relevant sample system to confirm species-specific detection.
• For ELISA applications, evaluate pairing with an appropriate capture antibody and optimize assay conditions; recombinant PKCα protein can serve as a standard.
• Phosphorylation-dependent conformational changes may influence antibody binding; if studying activated PKCα, consider pre-treatment with phorbol esters (e.g., PMA) or other stimuli in cellular assays.

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CamelBio provides diagnostic manufacturers and research institutes with integrated access to IVD raw materials, supporting projects from discovery to commercialization. This anti-PKC alpha antibody, targeting a key kinase in cell signaling and oncology, exemplifies our commitment to supplying validated monoclonal antibodies, bulk ancillary reagents, and rare-target raw-material sourcing. Partner with CamelBio to streamline your assay development with reliable, one-stop procurement solutions.