Anti-PKC alpha Rabbit Polyclonal Antibody for WB, IHC-P, ELISA - P17252

Core Product Specifications and Parameters

Biological Background: PKC alpha Function and Localization

• Protein kinase C alpha (PKCα) is a calcium-activated, phospholipid- and diacylglycerol (DAG)-dependent serine/threonine-protein kinase encoded by the PRKCA gene. It belongs to the PKC family and contains characteristic zinc-finger domains and an ATP-binding site.
• PKCα plays a central role in cell proliferation, differentiation, apoptosis, migration, and adhesion. It promotes cell growth via RAF1-mediated MAPK/ERK signaling and can also induce cell cycle arrest by upregulating CDK inhibitors CDKN1A and CDKN1B.
• Exhibits anti-apoptotic activity in cancer cells; for example, it phosphorylates BCL2 to block apoptosis and suppresses p53/TP53-mediated activation of IGFBP3 in glioma cells.
• Highly expressed in many cancers and functions as a tumor promoter, contributing to malignant phenotypes in gliomas and breast carcinomas.
• In the cardiovascular system, negatively regulates myocardial contractility through phosphorylation of cardiac troponin T (TNNT2/CTNT) and mediates cardiomyocyte hypertrophy via MAPK1/3 (ERK1/2) signaling.
• Involved in angiogenesis, platelet aggregation, and inflammation. It regulates endothelial cell migration and VEGFA signaling, and modulates LPS-induced macrophage responses.
• Subcellular localization includes cytoplasm, cell membrane, mitochondrion membrane, and nucleus. Stimulus-dependent translocation occurs, such as movement to lamellipodia upon wounding or to the nucleus during macrophage differentiation.
• Additional phosphorylation targets include DOCK8 (regulating T cell migration through CDC42 activation), KLHL3 (modulating WNK4 interaction in response to angiotensin II), and LRRK1 (controlling RAB protein trafficking). Related references: PMID:28028151 PMID:25313067 PMID:36040231

Experimental Guidance and Technical Tips

• The immunogen corresponds to a C-terminal region (aa 573–672) of human PKC alpha. This sequence is highly conserved across human, mouse, and rat, supporting the observed cross-reactivity.
• For Western blotting, consider starting dilutions between 1:500 and 1:2000 and validate on lysates from cell lines or tissues known to express PKCα (e.g., brain, heart, cancer cell lines).
• In IHC-P, antigen retrieval may be necessary; optimize fixation and retrieval conditions for your sample type. Include appropriate positive and negative controls.
• As a polyclonal antibody, lot-to-lot performance may vary; it is advisable to titrate each new lot and confirm specificity in the relevant model system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with streamlined access to high-quality IVD raw materials and technical support, covering every stage from concept to clinic. This anti-PKC alpha polyclonal antibody suits investigations into oncology, cardiovascular disease, and signal transduction. Beyond individual antibodies, CamelBio offers validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and sourcing for rare-target raw materials to accelerate your assay development and clinical translation.