Anti-PKC alpha Rabbit Polyclonal Antibody for WB, ELISA - P17252

Core Product Specifications and Parameters

Biological Background: PKC alpha Function and Localization

• PKC alpha (protein kinase C alpha type) is a calcium-activated, phospholipid- and diacylglycerol-dependent serine/threonine-protein kinase belonging to the PKC family. It regulates diverse cellular processes including proliferation, apoptosis, differentiation, migration, and adhesion.
• Plays dual roles in cell cycle control: promotes proliferation via RAF1/MAPK/ERK activation and CDKN1A upregulation, and also triggers cell cycle arrest with RB1 hyperphosphorylation and CDK inhibitor induction upon PMA stimulation.
• Exhibits anti-apoptotic functions in cancer: suppresses TP53-mediated IGFBP3 activation in glioma cells and phosphorylates BCL2 in leukemia cells. Highly expressed in various cancers acting as a tumor promoter.
• Subcellularly localizes to cytoplasm, cell membrane, mitochondrial membrane, and nucleus. Translocates to lamellipodia after wounding, modulating desmosomal adhesion and cell motility.
• During chemokine-induced CD4+ T cell migration, phosphorylates DOCK8, leading to CDC42 activation (PubMed:28028151). Related references: PMID:28028151
• In cardiomyocytes, mediates hypertrophic growth via MAPK1/3 pathway and phosphorylates TNNT2, reducing myocardial contractility. Essential for angiogenesis, endothelial migration, and VEGFA signaling.
• Phosphorylates KLHL3 in response to angiotensin II, disrupting the KLHL3-WNK4 interaction (PubMed:25313067); phosphorylates LRRK1 to regulate RAB-mediated intracellular trafficking (PubMed:36040231). Related references: PMID:25313067 PMID:36040231
• Functions in platelet aggregation via CD36/GP4 signals and integrin activation, and modulates macrophage inflammatory responses to LPS, sometimes negatively regulating NF-kappa-B.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide from the C-terminal region (amino acids 573–672) of human PKC alpha, which may offer isoform specificity; cross-reactivity with other PKC isoforms should be assessed in relevant assay systems.
• For Western blot, a band at approximately 77 kDa is anticipated; validate using appropriate positive controls (e.g., lysates from human, mouse, or rat cell lines known to express PKC alpha).
• For ELISA, optimize coating conditions and antibody titration to achieve desired sensitivity; consider using recombinant PKC alpha protein as a standard.
• Pre-adsorption with the immunizing peptide can serve as a useful negative control to confirm band specificity in WB.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutes with comprehensive access to IVD raw materials, supporting projects from concept to clinic. This PKC alpha antibody targets a key kinase implicated in oncology, cardiovascular disease, and immunology, making it valuable for assay development in these areas. Our supply capabilities include validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and tailored sourcing of rare targets to accelerate your diagnostic innovation.