Anti-PKC alpha Rabbit Monoclonal Antibody for WB/ELISA - P17252

Core Product Specifications and Parameters

Biological Background: Protein Kinase C alpha Function and Localization

• PKC alpha is a serine/threonine kinase that plays pivotal roles in cell proliferation, differentiation, apoptosis, migration, and adhesion by phosphorylating targets such as RAF1, BCL2, and CSPG4, or by modulating MAPK/ERK and RAP1GAP signaling cascades.
• It exhibits both tumor-promoting and tumor-suppressive functions depending on context; in gliomas and breast cancers, it promotes cell growth, while in certain cell types it can induce cell cycle arrest through RB1 and CDKN1A/B.
• Subcellular localization is dynamic: PKC alpha resides in the cytoplasm, but translocates to the cell membrane, mitochondrial membrane, or nucleus upon activation, enabling context-specific signaling.
• In immune cells, it regulates CD4+ T cell migration by phosphorylating DOCK8, leading to CDC42 activation. Related references: PMID:28028151
• In the cardiovascular system, PKC alpha negatively regulates myocardial contractility, mediates cardiomyocyte hypertrophy via ERK1/2, and promotes angiogenesis by stabilizing VEGFA mRNA and enhancing endothelial cell migration.
• Phosphorylates KLHL3 to regulate ion transport in response to angiotensin II. Related references: PMID:25313067
• Phosphorylates and activates LRRK1 to control intracellular trafficking. Related references: PMID:36040231
• Structural hallmarks include C2 and C1 domains responsible for calcium and DAG binding, and a kinase domain with ATP-binding site; post-translational modifications such as phosphorylation and acetylation regulate its activity.
• Listed as a proto-oncogene, PKC alpha is a zinc-finger phosphoprotein that integrates signals from growth factors, hormones, and neurotransmitters.

Experimental Guidance and Technical Tips

• For Western blot, expect a band at approximately 77 kDa; validate with appropriate positive controls (e.g., HeLa, A431 cell lysates) and molecular weight markers.
• This antibody is suitable for ELISA; we recommend optimizing antibody concentrations by titration with a known positive antigen.
• The antibody cross-reacts with human, mouse, and rat PKC alpha, making it broadly applicable for studies in these species.
• Shipped on ice bags; upon receipt, store at -20°C for long-term stability. Avoid repeated freeze-thaw cycles.
• Consider using standard blocking buffers (e.g., 5% non-fat dry milk) and verify specificity with appropriate negative controls and siRNA knockdown if needed.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your integrated partner for IVD raw-material sourcing, offering a comprehensive portfolio from validated antibody pairs to customized monoclonal antibodies for diagnostic and research applications. With deep expertise in kinase targets like PKC alpha, we provide high-quality reagents, bulk ancillary supplies, and technical support to streamline your assay development. Whether you need this anti-PKC alpha monoclonal antibody or rare target sourcing, CamelBio bridges the gap from concept to clinic.