Anti-PKC alpha Monoclonal Antibody for WB, IHC-P, IP, ELISA - P17252

Core Product Specifications and Parameters

Biological Background: PKC alpha Function and Localization

• Protein kinase C alpha type, encoded by the PRKCA gene, is a calcium-activated, phospholipid- and diacylglycerol-dependent serine/threonine-protein kinase.
• Plays pivotal roles in cell proliferation, differentiation, apoptosis, migration, and adhesion, acting as a proto-oncogene in many cancers.
• Directly phosphorylates targets like RAF1, BCL2, and CSPG4, and modulates signaling cascades including MAPK/ERK and RAP1GAP.
• During chemokine-induced CD4+ T cell migration, phosphorylates DOCK8, leading to dissociation from LRCH1 and activation of GTPase CDC42. Related references: PMID:28028151
• In response to angiotensin II signaling, phosphorylates KLHL3 at the cell membrane, decreasing KLHL3 interaction with WNK4. Related references: PMID:25313067
• Phosphorylates and activates LRRK1, which in turn phosphorylates RAB proteins involved in intracellular trafficking. Related references: PMID:36040231
• Subcellular localization includes cytoplasm, cell membrane, mitochondrial membrane, and nucleus, with translocation upon stimulation.
• Annotated with keywords: Zinc-finger, repeat structure, ATP-binding, and proto-oncogene; undergoes acetylation and phosphorylation modifications.

Experimental Guidance and Technical Tips

• For western blot (WB), a prominent band is expected near 77 kDa, consistent with the predicted molecular weight. Consider titrating antibody dilutions to optimize signal-to-noise ratios.
• In immunohistochemistry (IHC-P), antigen retrieval methods may enhance epitope accessibility; validate in positive control tissues expressing PKC alpha.
• For immunoprecipitation (IP), confirm compatibility with your target protein’s expression level and lysis conditions.
• In ELISA, the antibody can be used for direct antigen detection; consider a suitable coating buffer and detection system.
• The immunogen sequence (amino acids 573–672) is conserved across human, mouse, and rat, supporting the observed cross-reactivity. Nevertheless, always validate performance in the species and application of interest.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio connects diagnostic manufacturers, labs, and research institutes with a comprehensive portfolio of IVD raw materials and technical services. For PKC alpha-related projects—such as oncology biomarker assays or cardiovascular research—CamelBio offers validated monoclonal antibodies, recombinant proteins, and customized bulk sourcing solutions. From concept to clinic, we streamline your raw-material procurement for accelerated assay development.