Anti-PKC alpha + beta + gamma Monoclonal Antibody for WB, ELISA - P05771 / P17252 / P05129 / Q02156

Core Product Specifications and Parameters

Biological Background: PKC alpha + beta + gamma Function and Localization

• Protein kinase C beta (PKCβ) is a calcium-activated, phospholipid- and diacylglycerol (DAG)-dependent serine/threonine-protein kinase that plays a pivotal role in B-cell receptor (BCR) signal transduction. It mediates NF-kB activation by phosphorylating CARD11/CARMA1 and down-modulates BTK function via direct phosphorylation at Ser-180. Related references: PMID:11598012
• PKCβ acts as a coactivator of androgen receptor (AR)-dependent transcription by being recruited to AR target genes and specifically phosphorylating histone H3 at Thr-6 (H3T6ph), an epigenetic mark that prevents demethylation of H3K4me. Related references: PMID:20228790
• In insulin signaling, PKCβ phosphorylates glucose transporter SLC2A1/GLUT1 to promote glucose uptake, and it may function downstream of IRS1 to mediate DNA synthesis via the RAF1-MAPK/ERK pathway. Related references: PMID:25982116
• PKCβ phosphorylates ATF2, promoting cooperation between ATF2 and JUN and thereby activating transcription. Related references: PMID:19176525
• It phosphorylates KLHL3 in response to angiotensin II signaling, decreasing the interaction between KLHL3 and WNK4 and thus modulating ion homeostasis. Related references: PMID:25313067
• PKCβ phosphorylates and activates LRRK1, which in turn phosphorylates RAB proteins involved in intracellular trafficking. Related references: PMID:36040231
• PKCβ is an activator of ferroptosis: it phosphorylates ACSL4 to promote lipid peroxidation and phosphorylates FOXK1 to stimulate LGALS13 secretion, propagating ferroptosis signaling. Related references: PMID:35027735 PMID:40246981
• Subcellular localization includes cytoplasm, nucleus, and membrane. The antibody’s immunogen is derived from a conserved region shared by PKC alpha, beta, and gamma, enabling pan-isoform recognition across human, mouse, and rat.

Experimental Guidance and Technical Tips

• The immunogen peptide corresponds to a conserved sequence (aa 300–500) in PKC alpha, beta, and gamma, which may allow detection of multiple isoforms. Confirm specificity by using isoform-specific controls or knockdown/inhibitor studies if needed.
• For WB, consider a starting dilution range and optimize based on target abundance in your sample type; the predicted band size is approximately 78 kDa.
• In ELISA, the antibody can be used for capture or detection; pairing with an appropriate secondary antibody and validating in the relevant species matrix is recommended.
• Cross-reactivity has been observed with human, mouse, and rat samples; always include appropriate positive and negative controls when analyzing samples from other species.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is dedicated to supporting diagnostic manufacturers and research laboratories with a comprehensive portfolio of IVD raw materials. This pan-PKC rabbit monoclonal antibody is an ideal tool for studies in signal transduction, immune cell signaling, and metabolic regulation. Through our integrated sourcing services, we offer validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and rare-target raw material procurement—all designed to streamline your development from concept to clinic.