Anti-PKACα Rabbit Polyclonal Antibody for WB and ELISA - P17612

Core Product Specifications and Parameters

Biological Background: PKACα Function and Localization

• PKACα (PRKACA) is the catalytic subunit alpha of cAMP-dependent protein kinase. Two isoforms exist: ubiquitous isoform 1 and sperm-specific isoform 2 enriched in pachytene spermatocytes.
• It phosphorylates numerous cytoplasmic and nuclear substrates, including CDC25B, ABL1, NFKB1, histone H1.4, and VASP, regulating cell cycle, transcription, and motility. Related references: PMID:15642694, PMID:15905176, PMID:16387847
• In platelets, PKACα complexes with NF-κB and IκBα to enforce quiescence; agonist stimulation dissociates the complex, freeing PKACα to phosphorylate VASP and promote aggregation. Related references: PMID:15642694, PMID:20356841
• PKACα inhibits smoothened signaling by phosphorylating GLI transcription factors (GLI1/2/3), triggering their processing and blocking target gene expression in the absence of hedgehog ligands. Related references: PMID:16705181
• Sequestration of PKACα by the Smoothened receptor at the cell membrane prevents GLI phosphorylation, allowing pathway activation. Related references: PMID:36202993, PMID:39138140
• PKACα potentiates RYR2 calcium channel activity, altering calcium release dynamics and contributing to store overload-induced Ca²⁺ release. Related references: PMID:17693412
• It negatively regulates mTORC1 by phosphorylating RPTOR, linking cAMP signaling to nutrient sensing. Related references: PMID:31112131
• Subcellularly, PKACα is found in the cytoplasm, nucleus, cell membrane, mitochondria, and cell projections (cilia/flagella), reflecting its multifaceted functions.

Experimental Guidance and Technical Tips

• The immunogen corresponds to a conserved region (amino acids 251-351) within the catalytic domain; cross-reactivity with other PKA catalytic subunits (PRKACB, PRKACG) is possible, so verify specificity in your system.
• In Western blot, a major band at ~39–40 kDa is anticipated; additional bands may represent isoforms or degradation products. Use appropriate positive and negative controls.
• For ELISA, titrate both coating antigen and antibody to optimize signal-to-noise ratio; consider using recombinant PKACα fragment as a standard.
• Given its role in multiple signaling pathways, treat samples with phosphatase inhibitors to preserve phosphorylation-dependent interactions if studying such contexts.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. This anti-PKACα antibody supports research into cAMP/PKA signaling, kinase activity, and related disorders such as endocrine and cardiovascular diseases. Our portfolio includes validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare target raw-material sourcing to accelerate your development.