Anti-PKA C-beta (PRKACB) Rabbit Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - P22694

Core Product Specifications and Parameters

Biological Background: PKA C-beta (PRKACB) Function and Localization

• Mediates cAMP-dependent signaling triggered by receptor binding to GPCRs, serving as a catalytic subunit of PKA. Related references: PMID:12420224, PMID:21423175, PMID:31112131

• PKA activation regulates diverse cellular processes such as cell proliferation, cell cycle, differentiation, microtubule dynamics, chromatin condensation/decondensation, nuclear envelope dynamics, intracellular transport, and ion flux. Related references: PMID:12420224, PMID:21423175

• Regulates the abundance of compartmentalized regulatory subunits by phosphorylating PJA2, which ubiquitinates them and triggers proteolysis. Related references: PMID:12420224, PMID:21423175

• Phosphorylates GPKOW, modulating its RNA-binding ability. Related references: PMID:21880142

• Acts as a negative regulator of mTORC1 by phosphorylating RPTOR. Related references: PMID:31112131

• Subcellular localization includes cytoplasm, cell membrane, and nucleus.

• Isoform 1 is most abundant in brain, with low expression in kidney; isoform 2 predominates in thymus, spleen, and kidney; isoforms 3 and 4 are only expressed in brain.

• Annotated with alternative splicing, ATP-binding, kinase, myristoylation, and serine/threonine-protein kinase activities.

Experimental Guidance and Technical Tips

• The immunogen corresponds to residues 250–351; for WB, expect a band at ~41 kDa (calculated MW ~40.6 kDa). Validate with positive controls such as brain or kidney lysates.
• For IHC-P, test antigen retrieval conditions and confirm staining pattern in tissues with known expression (e.g., brain, kidney).
• In IF/ICC, observe anticipated cytoplasmic, membranous, or nuclear localization and consider peptide blocking to verify specificity.
• Cross-reactivity with mouse and rat is indicated; confirm performance in your specific model system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers and research laboratories with a comprehensive range of IVD raw materials and technical resources. This anti-PRKACB antibody complements our kinase signaling portfolio, and we offer validated antibody pairs, cost-optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and custom sourcing for rare targets. Contact us to streamline your assay development from concept to clinic.