Anti-PKA C-alpha (PRKACA) Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - P17612

Core Product Specifications and Parameters

Biological Background: PKA C-alpha (PRKACA) Function and Localization

• PRKACA encodes the catalytic subunit alpha of cAMP-dependent protein kinase (PKA), a major Ser/Thr kinase that transduces cAMP signals. Isoform 1 is ubiquitously expressed, while isoform 2 is sperm-specific.

• The kinase phosphorylates a vast array of cytoplasmic and nuclear substrates, including transcription factors (NFKB1, SOX9), ion channels (RYR2), signaling proteins (VASP, ABL1), and histones (H1.4), thereby regulating cell cycle, metabolism, and gene expression. Related references: PMID:15642694 PMID:15905176 PMID:16387847

• In platelets, PRKACA maintains quiescence by forming inhibitory complexes with NF-kappa-B and I-kappa-B-alpha; disruption by thrombin or collagen leads to VASP phosphorylation and platelet aggregation. Related references: PMID:15642694 PMID:20356841

• PRKACA acts as a key negative regulator of the smoothened signaling pathway by phosphorylating GLI transcription factors (GLI1/2/3), promoting their proteolytic processing and transcriptional inactivation; this inhibition is relieved when SMO sequesters PRKACA at the plasma membrane. Related references: PMID:16705181 PMID:36202993 PMID:39138140

• The protein localizes to multiple subcellular compartments, including cytoplasm, nucleus, cell membrane, mitochondria, and cilia/flagellum, reflecting its diverse signaling roles.

• Additional regulatory functions include phosphorylation of HSF1 to promote heat-shock response, modulation of mTORC1 via RPTOR phosphorylation, and phosphorylation of AKAP19. Related references: PMID:21085490 PMID:31112131 PMID:27028580

• Disease-associated mutations in PRKACA are linked to Cushing syndrome, underscoring its endocrine importance.

Experimental Guidance and Technical Tips

• The immunogen spans nearly the full-length human PRKACA protein (amino acids 1-351), suggesting broad epitope recognition. For Western blot, expect a main band at ~41 kDa. Verify specificity using PRKACA knockdown or knockout cell lysates.

• This polyclonal antibody is suitable for immunofluorescence/immunocytochemistry; consider optimizing fixation and permeabilization protocols to detect the diverse subcellular pools (cytoplasmic, nuclear, membrane, mitochondrial).

• The antibody cross-reacts with mouse and rat PRKACA, enabling studies in rodent models. Validate species-specific reactivity in your sample system before quantitative assays.

• For ELISA, the antibody can serve as a capture or detection reagent; test pairing with other anti-PRKACA antibodies to develop high-sensitivity sandwich ELISA.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and laboratories with one-stop access to validated raw materials for IVD development, including high-quality polyclonal antibodies like this anti-PRKACA pAb. Our sourcing expertise covers rare targets and bulk ancillary reagents, supporting your cAMP/PKA pathway research and kinase assay development. From antibody pairs to technical consultation, CamelBio streamlines your journey from concept to clinic.