Anti-PKA C-alpha (PRKACA) Monoclonal Antibody (KD Validated) for WB, IF/ICC, ELISA - P17612

Core Product Specifications and Parameters

Biological Background: PKA C-alpha (PRKACA) Function and Localization

• PRKACA encodes the catalytic subunit alpha of cAMP-dependent protein kinase (PKA), a serine/threonine kinase that phosphorylates numerous cytoplasmic and nuclear substrates, playing a central role in cAMP signaling cascades (PubMed:15642694, PubMed:15905176, PubMed:16387847). Related references: PMID:15642694 PMID:15905176 PMID:16387847

• The enzyme modulates chromatin structure and transcription by phosphorylating histone H1.4 and the NF-kappa-B p50 subunit (NFKB1), influencing gene expression and DNA binding activity (PubMed:15178447, PubMed:15642694, PubMed:15905176). Related references: PMID:15178447 PMID:15642694 PMID:15905176

• In platelets, PRKACA phosphorylates VASP to maintain circulating platelets in a resting state; upon disruption by thrombin or collagen, free active PRKACA promotes platelet aggregation (PubMed:15642694, PubMed:20356841). Related references: PMID:15642694 PMID:20356841

• PRKACA acts as a key inhibitor of the smoothened signaling pathway by phosphorylating GLI transcription factors (GLI1, GLI2, GLI3), promoting their processing and preventing target gene activation in absence of hedgehog ligands (PubMed:16705181, PubMed:36202993, PubMed:39138140). Related references: PMID:16705181 PMID:36202993 PMID:39138140

• Subcellularly, PRKACA localizes to the cytoplasm, nucleus, cell membrane, mitochondria, and specific projections like cilia and flagella, reflecting its diverse regulatory roles. Tissue expression is isoform-specific: isoform 1 is ubiquitous, while isoform 2 is sperm-specific and enriched in pachytene spermatocytes.

• Post-translational modifications including N-terminal myristoylation and phosphorylation regulate its catalytic activity, subcellular targeting, and stability. Alternative splicing gives rise to the distinct isoforms.

Experimental Guidance and Technical Tips

• This antibody was generated using recombinant human PRKACA protein; it cross-reacts with human, mouse, and rat, making it suitable for studies across these species. Validate endogenous expression levels in your model system before quantitative analyses.
• For WB, the expected molecular weight is approximately 41 kDa; include appropriate positive and negative controls (e.g., PRKACA-overexpressing and siRNA-treated lysates) to confirm band specificity.
• For IF/ICC, consider fixation/permeabilization protocols that preserve antigenicity; expect cytoplasmic and nuclear staining patterns consistent with reported localization.
• For ELISA, titrate the antibody for optimal signal-to-noise ratio and pair with a suitable secondary antibody; validate performance using recombinant protein or cell lysates.
• KD validated status indicates that antibody specificity has been confirmed using siRNA- or shRNA-mediated knockdown approaches; consider including knockdown controls in your assays to further verify signal dependency on target expression.

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CamelBio provides diagnostic manufacturers and research laboratories with a comprehensive portfolio of IVD raw materials, including validated antibodies like this anti-PKA C-alpha (PRKACA) monoclonal antibody for cAMP signaling and kinase research. Our one-stop sourcing bridges the gap from concept to clinic, offering optimized antibody pairs, bulk ancillary reagents, and custom rare-target raw material sourcing to accelerate your assay development and procurement workflows.