Anti-PKA C-alpha (PRKACA) Monoclonal Antibody (KD Validated) for WB, ELISA - P17612

Core Product Specifications and Parameters

Biological Background: PKA C-alpha (PRKACA) Function and Localization

• PRKACA encodes cAMP-dependent protein kinase catalytic subunit alpha, a serine/threonine kinase. Two isoforms exist: ubiquitous isoform 1 and sperm-specific isoform 2, enriched in pachytene spermatocytes.
• Phosphorylates a broad range of substrates in the cytoplasm and nucleus, regulating cell cycle progression, transcription, and apoptosis. Related references: PMID:15642694, PMID:15905176, PMID:16387847
• Regulates platelet function: maintains resting state via NF-kappa-B complex, while thrombin/collagen activate PRKACA to phosphorylate VASP, promoting platelet aggregation. Related references: PMID:15642694, PMID:20356841
• Key inhibitor of smoothened signaling: phosphorylates GLI transcription factors, promoting their processing and silencing target genes; interaction with SMO sequesters PRKACA at the membrane. Related references: PMID:16705181, PMID:36202993, PMID:39138140
• Localizes to diverse compartments: cytoplasm, nucleus, cell membrane, mitochondrion, cilia, and secretory vesicles.
• Post-translational modifications include N-terminal myristoylation and multiple phosphorylation sites, influencing activity and localization.
• Disease association: mutations linked to Cushing syndrome (PPNAD4).

Experimental Guidance and Technical Tips

• The immunogen corresponds to full-length recombinant human PRKACA; this design may provide comprehensive epitope coverage and support detection of both isoforms and cross-reactivity with mouse and rat.
• For Western blot, the predicted molecular weight is ~41 kDa; validate with appropriate positive controls (e.g., HeLa, HEK293, or tissues expressing PRKACA).
• For ELISA, consider pairing with a detection antibody targeting a non-overlapping epitope to develop sandwich assays.
• Knockdown validation (KD Validated) indicates the antibody can be used to confirm target specificity via siRNA/shRNA knockdown experiments; include relevant knockdown controls.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and labs with a one-stop sourcing bridge for high-quality IVD raw materials. This anti-PKA C-alpha antibody supports the development of kinase signaling assays and can be sourced along with matched antibody pairs, recombinant antigens, and bulk ancillary reagents. Our team assists with material customization and rare target sourcing to accelerate your IVD projects from concept to clinic.