Anti-PJVK Rabbit Monoclonal Antibody for WB, ELISA - Q0ZLH3

Core Product Specifications and Parameters

Biological Background: Pejvakin Function and Localization

• Pejvakin (PJVK), also known as autosomal recessive deafness type 59 protein (DFNB59), is encoded by the PJVK gene in humans.
• It is a peroxisome-associated protein critical for protecting auditory hair cells against noise-induced damage by regulating peroxisome proliferation and pexophagy in response to oxidative stress.
• PJVK acts as a reactive oxygen species (ROS) sensor; upon noise overexposure, it recruits the autophagy machinery to degrade damaged peroxisomes, preserving cellular integrity.
• The protein localizes to the peroxisome membrane and is also found in cell projections such as cilia, aligning with its role in sensory hair cells.
• Mutations in PJVK are linked to non-syndromic deafness, underscoring its essential role in hearing.
• The immunogen corresponds to amino acids 290-352, a region that may be involved in protein-protein interactions or targeting to peroxisomes.

Experimental Guidance and Technical Tips

• For Western blotting, the predicted molecular weight is approximately 40 kDa; validate detection in human cell lines or tissues expressing pejvakin, such as inner ear-derived cells.
• Due to the peroxisomal localization, consider using appropriate fractionation or whole-cell lysates with mild detergents to preserve membrane protein solubility.
• In ELISA applications, the antibody may be used as a capture or detection reagent; titrate and optimize against recombinant pejvakin or cell lysates.
• Since pejvakin expression may be induced by oxidative stress, treatment of cells with ROS-generating agents before lysis could enhance signal in certain experimental setups.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers and hearing research labs with a comprehensive portfolio of IVD raw materials, including validated antibody pairs, optimized monoclonal antibodies like this anti-PJVK clone, and bulk ancillary reagents. We streamline procurement for rare targets such as pejvakin, offering reliable sourcing and technical consulting to accelerate assay development from concept to clinic.