Anti-PJVK Rabbit Monoclonal Antibody for WB, ELISA - Q0ZLH3

Core Product Specifications and Parameters

Biological Background: PJVK (Pejvakin) Function and Localization

• PJVK (pejvakin), also known as autosomal recessive deafness type 59 protein (DFNB59), is a peroxisome-associated protein encoded by the PJVK gene in humans.
• It plays a critical role in protecting auditory hair cells and neurons from noise-induced damage by regulating peroxisome dynamics.
• Acts as a ROS sensor, and upon noise overexposure, it recruits autophagy machinery to trigger pexophagy, the selective degradation of damaged peroxisomes.
• Also required for promoting peroxisome proliferation in response to sound overstimulation, maintaining cellular homeostasis.
• Localizes to the peroxisome membrane and is also detected at cell projections and cilia, suggesting a role in sensory organelle function.
• Mutations in PJVK are associated with non-syndromic hearing loss (DFNB59), highlighting its essential role in auditory function.
• The protein has a molecular weight of approximately 40 kDa (39913 Da as per UniProt), consistent with its detection in WB.

Experimental Guidance and Technical Tips

• For Western blotting, a specific band around 40 kDa is expected. Validate in appropriate human cell lines or tissues expressing PJVK, such as inner ear hair cells or peroxisome-rich samples.
• For ELISA applications, consider using the antibody as a capture or detection reagent; proper pairing and validation are essential for assay development.
• The immunogen covers a C-terminal region (aa 290–352), so the antibody likely recognizes epitopes within this segment; cross-check with other antibodies if full-length recognition is required.
• As PJVK is a peroxisomal membrane protein, include mild detergents in lysis buffers to ensure solubilization while preserving epitope integrity.
• While not listed as an application, immunofluorescence detection is possible after fixation and permeabilization, but thorough in-house validation is advised.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a dedicated IVD raw-material sourcing partner, providing diagnostic manufacturers, laboratories, and research institutes with integrated access to high-quality antibodies, recombinant proteins, and ancillary reagents. For hearing loss and peroxisome biology research, we offer validated monoclonal antibodies like this anti-PJVK antibody, along with optimized antibody pairs, bulk ordering options, and custom sourcing for rare targets. From concept to clinic, CamelBio streamlines your raw-material procurement for diagnostic and research applications.