Anti-PIWIL2 Rabbit Polyclonal Antibody for WB and ELISA - Q8TC59

Core Product Specifications and Parameters

Biological Background: PIWIL2 Function and Localization

• PIWIL2 (Piwi-like protein 2) is an endoribonuclease that plays a central role in spermatogenesis by repressing transposable elements via the piRNA pathway (By similarity).
• Acts as a 'slicer-competent' piRNA endoribonuclease in the ping-pong amplification loop, cleaving primary piRNAs to produce mature piRNAs and facilitating PIWIL4 nuclear localization and secondary piRNA loading (By similarity).
• Essential for meiotic differentiation of spermatocytes and self-renewal of spermatogonial stem cells, indirectly modulating expression of genes like PDGFRB, SLC2A1, and STRA8 (By similarity).
• Overexpression of PIWIL2 inhibits apoptosis and promotes proliferation in tumors, acting as an oncogene. Related references: PMID:16377660
• Represses circadian rhythms by stabilizing core clock components BMAL1 and CLOCK, inhibiting GSK3B-mediated phosphorylation and degradation. Related references: PMID:28903391
• Subcellularly localized in the cytoplasm (UniProt Q8TC59).
• Expressed in adult testis and in most tumors, highlighting its role in germline integrity and cancer.
• Belongs to the Piwi family of Argonaute proteins, characterized by RNA-binding and metal-binding properties.

Experimental Guidance and Technical Tips

• The immunogen corresponds to a peptide within amino acids 100-200 of human PIWIL2 (Q8TC59); for Western blot, consider using testis or tumor cell lysates as positive controls given the tissue-specific expression.
• Cross-reactivity with mouse is confirmed; this antibody may be suitable for mouse spermatogenesis and piRNA pathway studies.
• For ELISA, validate coating and detection conditions empirically, and confirm specificity with appropriate controls.
• Ensure proper handling: shipped on ice bags to maintain stability upon receipt.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. For your PIWIL2 research in spermatogenesis, piRNA biology, and germline integrity, we offer validated antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk ancillary reagents to support assay development and rare target raw-material sourcing. Let CamelBio be your trusted partner in accelerating diagnostic and research workflows.