Anti-PIWIL1 Polyclonal Antibody for WB, IF-P, IHC-P, ELISA - Q9JMB7

Core Product Specifications and Parameters

Biological Background: PIWIL1 Function and Localization

• Piwi-like protein 1 (PIWIL1/MIWI) is an endoribonuclease that plays a central role in postnatal germ cells by repressing transposable elements, thereby safeguarding germline integrity. Related references: PMID:11578866, PMID:21237665, PMID:22121019

• As a core component of the piRNA pathway, PIWIL1 directly binds methylated piRNAs with a strong preference for uridine at the first position (1U-bias), guiding transposon methylation and silencing. Related references: PMID:24757166, PMID:11578866, PMID:21237665, PMID:22121019

• PIWIL1 cleaves transposon mRNAs via its endoribonuclease activity but is not involved in the piRNA amplification (ping-pong) cycle. Related references: PMID:22121019

• The protein localizes to the cytoplasm and exhibits tissue-specific expression in the testis (spermatocytes) and brain. In male gonads, expression initiates around two weeks after birth coinciding with spermatogenesis onset.

• PIWIL1 is required for chromatoid body formation and contributes to the stability of certain miRNAs, suggesting additional regulatory roles beyond transposon silencing. Related references: PMID:16787948

• During late spermatogenesis, PIWIL1 sequesters RNF8 in the cytoplasm; its own ubiquitination and subsequent degradation release RNF8, enabling histone-to-protamine exchange. Related references: PMID:28552346

• Key functional features include metal ion cofactor binding (magnesium), alternative splicing, and post-translational modifications (ubiquitination, methylation), classifying PIWIL1 as a multi‑domain regulatory hydrolase.

Experimental Guidance and Technical Tips

• The immunogen (N-terminal amino acids 1–160) lies within a region conserved across human, mouse, and rat, aligning with the reported cross-reactivity profile; consider verifying reactivity in your specific model system.
• For Western blot, a band at approximately 99 kDa is expected. Use a positive control such as mouse testis lysate and validate the observed band with an appropriate loading control.
• Immunofluorescence detection requires permeabilization to access the cytoplasmic localization; co-staining with germ cell markers (e.g., DDX4) can help confirm cell-type specificity.
• For immunohistochemistry on formalin-fixed paraffin-embedded sections, antigen retrieval may be necessary; testicular tissue sections serve as a suitable positive control.
• ELISA applications may benefit from using a peptide or fragment‑based coating antigen; titrate antibody concentrations to optimize signal-to-noise ratio.

CamelBio: Your One-Stop Sourcing Bridge

As a dedicated IVD raw‑material partner, CamelBio simplifies procurement for diagnostic developers and researchers working on PIWIL1, the piRNA pathway, and germ cell biology. We can supply validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and facilitate sourcing of rare‑target raw materials—all with the technical support needed to advance your projects from concept to clinic.