Anti-PIWIL1 Monoclonal Antibody for WB, IF-P, IHC-P, ELISA - Q96J94

Core Product Specifications and Parameters

Biological Background: PIWIL1 Function and Localization

• PIWIL1 (Piwi-like protein 1) is an endoribonuclease that plays a central role in postnatal germ cells by repressing transposable elements via the piRNA pathway, essential for germline integrity.
• Acts as an endoribonuclease that cleaves transposon messenger RNAs; strongly prefers a uridine in the first position of its guide piRNAs (1U-bias).
• May function as a negative developmental regulator. Related references: PMID:12037681 PMID:16287078
• Localizes to the cytoplasm and is required for the formation of chromatoid bodies.
• Expressed specifically in spermatocytes and spermatids in the testis; also detected in prostate cancer and overexpressed in gastric cancer cells. Isoform 3 is ubiquitously expressed, including in CD34+ hematopoietic progenitor cells.
• Binds methylated piRNAs and is not involved in the piRNA ping-pong amplification cycle.
• Required for the stability of some miRNAs and sequesters RNF8 in the cytoplasm until late spermatogenesis; degraded by ubiquitination.
• Annotated with keywords: Developmental protein, Differentiation, Meiosis, RNA-mediated gene silencing, Spermatogenesis.

Experimental Guidance and Technical Tips

• The immunogen corresponds to a sequence within amino acids 700–800 of human PIWIL1; consider this region for epitope specificity when comparing with other antibodies.
• The antibody is suitable for western blotting (predicted band ~99 kDa), immunofluorescence (IF-P), immunohistochemistry (IHC-P), and ELISA. Validate in appropriate tissue or cell systems expressing PIWIL1, such as testis or gastric cancer samples.
• Cross-reactivity with mouse and rat has been reported; verify reactivity in your specific experimental context.
• For immunofluorescence, note that PIWIL1 localizes to the cytoplasm, so expect cytoplasmic staining patterns.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research labs with streamlined access to validated antibody pairs, optimized monoclonal antibodies, and ancillary reagents for reproductive biology and oncology targets like PIWIL1. From bulk antibody supply to rare target raw-material sourcing, our team supports your IVD development from concept to clinic with reliable procurement solutions.