Anti-PITX3 Rabbit Monoclonal Antibody for WB, ELISA - O75364

Core Product Specifications and Parameters

Biological Background: PITX3 Function and Localization

• PITX3 is a paired-like homeodomain transcription factor critical for the differentiation and long-term survival of meso-diencephalic dopaminergic (mdDA) neurons.
• It activates NR4A2/NURR1-mediated transcription of genes like SLC6A3, TH, and DRD2 by displacing the corepressor NCOR2/SMRT from promoters.
• PITX3 is essential for normal lens development, regulating lens epithelial cell proliferation and fiber cell differentiation.
• In the lens, PITX3 positively regulates FOXE3 and negatively regulates PROX1, preventing premature expression of CDKN1B/P27Kip1 and CDKN1C/P57Kip2 to maintain the cell cycle.
• Localizes exclusively to the nucleus, consistent with its DNA-binding and transcriptional regulatory functions.
• Contains a homeobox domain and is subject to phosphorylation, as indicated by proteomics studies.
• Mutations in PITX3 are associated with congenital cataracts and anterior segment mesenchymal dysgenesis (ASMD).
• Highly expressed in developing eye lens, with additional roles in dopaminergic neuron populations.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal region (amino acids 1-100) of human PITX3; consider the epitope location when designing downstream assays.
• For Western blot, a band around 32 kDa is expected; validate using appropriate positive controls (e.g., lens or dopaminergic neuron lysates) and consider post-translational modifications that may alter migration.
• ELISA protocols can utilize the antibody as a capture or detection reagent; optimize coating concentrations for indirect ELISA setups.
• Cross-reactivity with mouse and rat makes this antibody suitable for studies in rodent models, but always verify species-specific reactivity in your experimental system.
• Due to the monoclonal nature, lot-to-lot consistency is expected; still, perform routine quality control checks upon receipt.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio streamlines IVD raw-material procurement for diagnostic manufacturers, labs, and research institutes, offering a comprehensive portfolio from concept to clinic. This anti-PITX3 monoclonal antibody supports neurodevelopment and ophthalmology research, and we can further supply validated antibody pairs, bulk ancillary reagents, and sourcing of rare targets. Partner with CamelBio for integrated raw-material solutions tailored to your diagnostic pipeline.