Anti-PITX2 (Pituitary homeobox 2) Rabbit Polyclonal Antibody for WB, ELISA - Q99697

Core Product Specifications and Parameters

Biological Background: PITX2 Function and Localization

• PITX2 (pituitary homeobox 2) is a paired-like homeodomain transcription factor, also known as ARP1, RIEG, RIEG1, PtX2, or Solurshin, encoded by the PITX2 gene in humans.
• It plays a role in myoblast differentiation: when unphosphorylated, it interacts with ELAVL1 to stabilize CCND1 mRNA, promoting proliferation; AKT2-mediated phosphorylation disrupts this complex, leading to CCND1 mRNA decay and differentiation.
• PITX2 is critical for establishing left-right asymmetry during embryonic development, influencing organ positioning and morphogenesis.
• The protein contains a conserved homeobox DNA-binding domain that allows it to regulate target gene expression.
• Subcellular localization includes both the nucleus and cytoplasm; nucleocytoplasmic shuttling may modulate its activity in response to signaling cues.
• It is a phosphoprotein, with phosphorylation by AKT2 serving as a key regulatory switch between proliferation and differentiation.
• Mutations in PITX2 are associated with anterior segment dysgenesis disorders, including Peters anomaly, Rieger syndrome type 1, and other ocular developmental defects (ASGD4).
• Multiple isoforms are produced by alternative splicing, which may exhibit distinct tissue distributions and functions.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal region (amino acids 1–100) of human PITX2, which is predicted to be accessible in both native and denaturing conditions, supporting use in Western blot (WB) and ELISA.
• For WB, a band of approximately 35 kDa is expected; due to potential post-translational modifications or isoform variability, additional bands may be observed. Validate with appropriate positive and negative controls from relevant cell lines or tissues.
• Cross-reactivity with mouse and rat has been reported; however, sequence alignment between species is recommended to confirm epitope conservation for your specific application.
• As a rabbit polyclonal antibody, it may recognize multiple isoforms and post-translational modifications, offering broader detection but requiring careful validation for specificity.
• For ELISA, consider using peptide-based coating antigens or recombinant PITX2 protein; optimization of coating buffers and antibody dilutions is advised.
• Include untreated and AKT2-stimulated samples to assess phospho-sensitive conformational changes if studying the AKT2 regulatory axis.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with integrated access to a wide spectrum of IVD raw materials. In the field of developmental biology and transcription factor research, we offer validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and specialized rare-target sourcing. This anti-PITX2 polyclonal antibody exemplifies our commitment to supplying high-quality materials that support studies on left-right asymmetry, myogenesis, and related disease diagnostics.