Anti-Pituitary homeobox 2 (PITX2) Rabbit Monoclonal Antibody for WB, ELISA - Q99697

Core Product Specifications and Parameters

Biological Background: PITX2 Function and Localization

• PITX2 is a paired-like homeodomain transcription factor also known as ALL1-responsive protein ARP1, homeobox protein PITX2, RIEG bicoid-related homeobox transcription factor, and solurshin.
• It plays a crucial role in embryonic development, particularly in the establishment of left-right asymmetry.
• PITX2 is involved in myoblast differentiation: when unphosphorylated, it associates with ELAVL1 to stabilize CCND1 mRNA, promoting cell proliferation; phosphorylation by AKT2 disrupts this complex, leading to mRNA destabilization and differentiation progression.
• The protein localizes to both the nucleus and cytoplasm.
• Alternative splicing generates multiple isoforms, and the protein is subject to phosphorylation.
• Mutations in PITX2 are associated with Peters anomaly and other developmental disorders (keywords include disease variant, Peters anomaly).

Experimental Guidance and Technical Tips

• For Western blotting, based on the predicted protein weight of 35 kDa, ensure appropriate gel percentage and transfer conditions to detect bands in the expected range.
• Validate the antibody in your specific sample system, as reactivity confirmed for human species only; negative control lysates may help confirm specificity.
• For ELISA, consider optimizing coating antigen and antibody concentration; the synthetic peptide immunogen may serve as a positive control.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting. We support your PITX2-related research and assay development with validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare target raw-material sourcing. Our streamlined procurement process ensures timely delivery for projects in developmental biology and congenital anomaly diagnostics.