Anti-PITRM1 Polyclonal Antibody for WB, IF/ICC, ELISA - Q5JRX3

Core Product Specifications and Parameters

Biological Background: PITRM1 Function and Localization

• PITRM1 is a mitochondrial matrix zinc metalloprotease belonging to the pitrilysin family, also designated as presequence protease (PreP) or metalloprotease 1 (hMP1).
• It functions as an ATP-independent metalloendopeptidase that degrades peptides ranging from 5 to 65 residues. Cleavage occurs preferentially after small polar residues and before basic residues, with no strict positional preference. Related references: PMID:10360838, PMID:19196155, PMID:24931469
• The enzyme processes mitochondrial transit peptides after their cleavage and degrades other unstructured peptides, playing a key role in mitochondrial peptide clearance. Related references: PMID:19196155
• Notably, PITRM1 degrades amyloid-beta peptides (Aβ40 and Aβ42) that accumulate in mitochondria, thus linking its function to mechanisms of neurodegeneration. Related references: PMID:16849325, PMID:24931469, PMID:26697887, PMID:29383861, PMID:29764912
• Subcellularly, PITRM1 localizes to the mitochondrial matrix, consistent with its role in peptide degradation within this organelle.
• Widely expressed, with higher levels in muscle and heart compared to brain, pancreas, liver, lung, and placenta.
• The protein undergoes acetylation and contains a transit peptide for mitochondrial import; alternative splicing yields multiple isoforms.

Experimental Guidance and Technical Tips

• The immunogen spans the C-terminal region (aa 758–1037) of human PITRM1, which may help avoid cross-reactivity with closely related pitrilysin family members; consider this epitope location when designing complementary detection reagents.
• For Western blotting, expect a specific band around 117 kDa in lysates from human, mouse, or rat samples; validate band identity using knockdown or knockout controls.
• In immunofluorescence/ICC, PITRM1 should display a mitochondrial staining pattern; co-localization with a mitochondrial marker is recommended.
• For ELISA applications, determine optimal antibody dilution and coating antigen concentrations empirically using recombinant PITRM1 or relevant biological samples.

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