Anti-PITRM1 Monoclonal Antibody for WB, IHC-P, IF/ICC, ELISA - Q5JRX3

Core Product Specifications and Parameters

Biological Background: PITRM1 Function and Localization

• PITRM1 (Presequence Protease, Mitochondrial) is an ATP-independent metalloendopeptidase of the mitochondrial matrix that functions primarily in peptide degradation, cleaving substrates in the 5–65 residue range. Related references: PMID:10360838, PMID:16849325, PMID:19196155
• The protease shows cleavage preference after small polar residues and before basic residues, without positional specificity, indicating broad substrate recognition. Related references: PMID:10360838, PMID:19196155, PMID:24931469
• A critical function involves degrading mitochondrial transit peptides after their proteolytic removal, as well as other unstructured peptides that enter the organelle. Related references: PMID:19196155
• PITRM1 efficiently degrades amyloid-beta (Aβ) peptides, including Aβ40 and Aβ42, which are implicated in Alzheimer disease, suggesting a protective role against mitochondrial accumulation of neurotoxic peptides. Related references: PMID:16849325, PMID:24931469, PMID:26697887, PMID:29383861, PMID:29764912
• Subcellularly, the protein localizes to the mitochondrion and is enriched in the mitochondrial matrix, consistent with its role in peptide surveillance.
• PITRM1 is widely expressed, with relatively higher levels in muscle and heart compared to brain, pancreas, liver, lung, and placenta.
• Mutations in the PITRM1 gene are associated with autosomal recessive spinocerebellar ataxia type 30 (SCAR30), and the protein is categorized under disease variant and neurodegeneration keywords.

Experimental Guidance and Technical Tips

• For western blot detection, a band approximately 117 kDa is expected; consider using appropriate molecular weight markers and validate expression in the relevant cell or tissue lysates.
• The antibody is recommended for IHC-P and IF/ICC applications; fixation and permeabilization conditions should be optimized empirically for your specific sample type.
• The recombinant immunogen spanning the C-terminal region (aa 758–1037) may support detection across multiple assay formats; however, validation in the intended experimental system is advised.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers this high-quality anti-PITRM1 monoclonal antibody as part of a comprehensive suite of IVD raw materials for mitochondrial and neurodegenerative disease research. Our portfolio includes validated antibody pairs, bulk ancillary reagents, and tailored sourcing for rare targets, streamlining your diagnostic development from concept to commercialization. Contact our team to explore custom solutions and procurement options.