Anti-PITPNA Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - Q00169

Core Product Specifications and Parameters

Biological Background: PITPNA Function and Localization

• The protein is known as Phosphatidylinositol transfer protein alpha isoform (PITPNA), with aliases including PITPN, VIB1A, HEL-S-36, and PI-TPalpha.
• Catalyzes the transfer of phosphatidylinositol (PI) and phosphatidylcholine (PC) between biological membranes, playing a key role in lipid-mediated signaling and membrane trafficking. Related references: PMID:10531358 PMID:14962392 PMID:15522822
• Exhibits a distinct substrate preference for PI and PC species with shorter saturated or monounsaturated acyl chains (e.g., C16:1 > C16:0 > C18:1 > C18:0 > C20:4 for PC and C16:1 > C16:0 > C18:1 > C18:0 > C20:4 > C20:3 for PI). Related references: PMID:15522822 PMID:22822086
• Subcellularly, PITPNA is predominantly localized in the cytoplasm and cytosol, but can also be detected in the nucleus, consistent with its role in inter-organelle lipid exchange.
• Keywords associated with the protein include lipid transport, lipid-binding, acetylation; it has been identified in proteomics studies and structurally characterized by 3D-structure determination.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 211–270 in the C‑terminal region of human PITPNA. Verify conservation of this epitope in mouse and rat to align with the observed cross‑reactivity.
• For Western blotting (WB), an expected band near 32 kDa is predicted; validate expression in target cell lines or tissues that endogenously express PITPNA.
• In immunofluorescence (IF/ICC), optimize fixation and permeabilization protocols to account for both the cytosolic and nuclear localization of PITPNA.
• Although validated for ELISA, titration and buffer conditions should be empirically determined to achieve optimal signal‑to‑noise ratios for your specific assay setup.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive sourcing partner for IVD raw materials, connecting diagnostic manufacturers and research teams with high-quality antibody reagents like this anti-PITPNA polyclonal antibody. For studies in lipid transport, membrane biology, or related signaling pathways, we offer a broad portfolio including validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and sourcing support for rare targets. Let CamelBio streamline your procurement process from concept to clinic.