Anti-Pit1/POU1F1 Monoclonal Antibody for WB, IP, IF-P, IHC-P, ELISA - P28069

Core Product Specifications and Parameters

Biological Background: POU1F1 (Pit-1) Function and Localization

• POU1F1, also known as Pit-1 or GHF-1, is a pituitary-specific transcription factor encoded by the POU1F1 gene (Gene ID: 5449).
• It plays a critical role in anterior pituitary development, specifying lactotrope, somatotrope, and thyrotrope cell lineages.
• It specifically binds to the consensus sequence 5'-TAAAT-3' and activates transcription of growth hormone (GH) and prolactin (PRL) genes. Related references: PMID:22010633 PMID:26612202
• The protein contains a POU-specific domain and a homeobox DNA-binding domain, classifying it as a member of the POU domain transcription factor family.
• Subcellular localization is primarily nuclear, consistent with its role as a transcription factor.
• Post-translational modifications and alternative splicing produce multiple isoforms (UniProt keyword: Alternative splicing).
• Mutations in POU1F1 are associated with combined pituitary hormone deficiency (CPHD1) and dwarfism (UniProt keywords: Disease variant, Dwarfism).

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 1-150, covering the N-terminal region; consider that some isoforms or cleavage products may not be detected.
• For Western blotting, the predicted molecular weight is 33kDa; validate with positive controls from pituitary-derived cell lines or tissues.
• In immunoprecipitation, nuclear extraction protocols may enhance enrichment of this transcription factor.
• For immunohistochemistry (IHC-P), nuclear staining is anticipated; include appropriate isotype controls and antigen retrieval steps.
• For ELISA, this antibody can be used as a capture or detection reagent; optimal coating concentration and detection conditions should be determined empirically.
• Cross-reactivity with mouse and rat has been indicated; however, verify species specificity in your experimental system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and researchers with streamlined access to validated IVD raw materials, including this POU1F1 (Pit-1) monoclonal antibody for pituitary function studies. Our portfolio encompasses optimized antibody pairs, bulk ancillary reagents, and rare target sourcing—supported by technical consultation from concept to clinic. Partner with CamelBio to accelerate your assay development timelines.