Anti-PIST/GOPC Rabbit Polyclonal Antibody for WB, ELISA - Q9HD26

Core Product Specifications and Parameters

Biological Background: GOPC Function and Localization

• GOPC (Golgi-associated PDZ and coiled-coil motif-containing protein, also known as PIST, CAL, FIG) is a multidomain adaptor protein involved in intracellular protein trafficking and degradation. Related references: PMID:11707463, PMID:14570915, PMID:15358775
• It regulates the cell surface expression and activity of CFTR chloride channels and ASIC3 acid-sensing channels, influencing ion transport.
• GOPC directly interacts with and modulates trafficking of the ADR1B receptor. Related references: PMID:15358775
• Together with the E3 ubiquitin ligase MARCHF2, GOPC mediates ubiquitination and lysosomal degradation of CFTR, leading to intracellular retention. Related references: PMID:23818989
• GOPC has been implicated in the regulation of autophagy.
• Subcellularly, GOPC localizes to the cytoplasm, Golgi apparatus (including trans-Golgi network), postsynaptic density, and dendrites.
• The protein undergoes acetylation and alternative splicing, and its gene is involved in chromosomal rearrangements. GOPC is ubiquitously expressed in human tissues.

Experimental Guidance and Technical Tips

• The immunogen corresponds to a central region (aa 195-454) of human GOPC, encompassing PDZ and coiled-coil domains; this region is conserved across human, mouse, and rat, supporting the reported cross-reactivity.
• For WB, a specific band around 51kDa (predicted molecular weight 50.5kDa) is expected. Consider using positive lysates from human, mouse, or rat tissues/cell lines and optimizing antibody dilution and detection conditions.
• In ELISA, the antibody can detect recombinant or endogenous GOPC; validate performance with appropriate standards and detection reagents.
• Given GOPC's role in CFTR regulation, autophagy, and receptor trafficking, this antibody may be useful in mechanistic studies; for applications beyond WB and ELISA (e.g., IP or IF), independent validation is recommended.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive sourcing partner for IVD raw materials, supporting diagnostic manufacturers and research labs from concept to clinical application. For GOPC-related research and assay development, we offer validated polyclonal antibodies, optimized antibody pairs, bulk ancillary reagents, and tailored sourcing for rare or custom targets. Contact us to streamline your procurement and accelerate your project delivery.