Anti-PIST/GOPC Monoclonal Antibody for WB - Q9HD26

Core Product Specifications and Parameters

Biological Background: PIST/GOPC Function and Localization

• PIST/GOPC (Golgi-associated PDZ and coiled-coil motif-containing protein) is a ubiquitously expressed adaptor protein encoded by the GOPC gene. Alternate names include CAL (CFTR-associated ligand), FIG (fused in glioblastoma), and PIST (PDZ protein interacting specifically with TC10).
• Functions in intracellular protein trafficking and degradation, influencing the cell surface expression and stability of various transmembrane proteins. Related references: PMID:11707463 PMID:14570915 PMID:15358775
• Regulates CFTR chloride currents and acid-sensing ion channel ASIC3 currents by modulating their cell surface expression (By similarity).
• Regulates the intracellular trafficking of the ADR1B receptor. Related references: PMID:15358775
• Together with the E3 ubiquitin ligase MARCHF2, mediates ubiquitination and lysosomal degradation of CFTR. Related references: PMID:23818989
• Overexpression leads to CFTR intracellular retention and lysosomal degradation. Related references: PMID:11707463 PMID:14570915
• Predominantly localizes to the Golgi apparatus membrane and trans-Golgi network, but is also found in the cytoplasm, postsynaptic density, and dendritic projections.
• May play a role in autophagy (By similarity).

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal 1-100 amino acids of human PIST/GOPC. This region is conserved across human, mouse, and rat, supporting the reported cross-reactivity.
• For Western blotting, a band at approximately 51 kDa is expected. Validate specificity using positive and negative controls expressing or lacking the target.
• In immunohistochemistry (IHC-P), antigen retrieval conditions may require optimization. Given ubiquitous expression, many tissues are suitable as positive controls; nonetheless, validate in your specific sample system.
• For immunofluorescence (IF/ICC), permeabilization and fixation conditions should be optimized for Golgi and cytoplasmic detection.
• ELISA applications may benefit from pairing with a suitable detection antibody; consider checkerboard titrations to determine optimal working concentrations.
• Always include appropriate controls (e.g., siRNA knockdown or knockout cell lines) to confirm antibody specificity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio equips diagnostic manufacturers, research laboratories, and clinical institutes with one-stop access to high-quality IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. This anti-PIST/GOPC monoclonal antibody supports investigations into intracellular protein trafficking, protein degradation, and channel regulation, and is complemented by CamelBio’s extensive portfolio of validated antibody pairs, optimized recombinant proteins, and bulk ancillary reagents. For rare or custom targets within the membrane trafficking field, our sourcing expertise ensures a reliable, scalable supply chain tailored to your diagnostic development needs.