Anti-PIP4K2A Rabbit Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - P48426

Core Product Specifications and Parameters

Biological Background: PIP4K2A Function and Localization

• PIP4K2A (Phosphatidylinositol 5-phosphate 4-kinase type-2 alpha), also known as PIP5K2A or PIP5KII-alpha, is a lipid kinase that catalyzes the phosphorylation of phosphatidylinositol 5-phosphate (PtdIns5P) to phosphatidylinositol 4,5-bisphosphate (PtdIns(4,5)P2) (PubMed:23326584, PubMed:9367159). Related references: PMID:23326584, PMID:9367159
• It possesses both ATP- and GTP-dependent kinase activities (PubMed:26774281). Related references: PMID:26774281
• It regulates the levels of the signaling lipid PtdIns5P, which activates AKT in the cytosol and signals through ING2 in the nucleus (PubMed:18364242). Related references: PMID:18364242
• PIP4K2A is required for lysosome-peroxisome membrane contacts and intracellular cholesterol transport by modulating peroxisomal PtdIns(4,5)P2 levels (PubMed:29353240). Related references: PMID:29353240
• It plays a role in autophagy by promoting autophagosome-lysosome fusion and regulating cellular lipid metabolism (PubMed:31091439). Related references: PMID:31091439
• PIP4K2A negatively regulates insulin signaling through a catalytic-independent mechanism, in part by suppressing PIP5K-mediated synthesis of PtdIns(4,5)P2 and its conversion to PtdIns(3,4,5)P3 (PubMed:31091439). Related references: PMID:31091439
• The protein is widely expressed, with highest levels in the brain, but is notably absent in skeletal muscle and small intestine.
• Subcellularly, PIP4K2A localizes to the cell membrane, nucleus, lysosome, cytoplasm, and photoreceptor segments, reflecting its diverse functional roles.

Experimental Guidance and Technical Tips

• The immunogen covers the central region (aa 120-350) of human PIP4K2A, which is conserved across human, mouse, and rat, supporting the antibody's cross-reactivity. Consider validating detection in the relevant species with appropriate positive and negative controls.
• For Western blotting, the predicted molecular weight is 46 kDa; confirm band size using molecular weight markers and consider using cell or tissue lysates known to express PIP4K2A.
• For IHC-P and IF/ICC, optimize fixation and permeabilization conditions to visualize PIP4K2A at its reported subcellular locations (cell membrane, nucleus, cytoplasm). Include appropriate isotype controls.
• ELISA specificity indicates the antibody can be used for quantitative detection; pairing with a matched detection antibody may improve sensitivity in sandwich ELISA formats.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a streamlined one-stop channel for diagnostic manufacturers and researchers sourcing IVD raw materials. This anti-PIP4K2A antibody supports studies in phosphoinositide signaling, autophagy, and insulin signaling pathways. Beyond this polyclonal antibody, CamelBio provides access to validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare target raw-material sourcing—simplifying your transition from concept to clinic.