Anti-PINK1 Polyclonal Antibody (Rabbit) for WB, IF/ICC, IHC-P, ELISA - Q9BXM7

Core Product Specifications and Parameters

Biological Background: PINK1 Function and Localization

• PINK1 is a serine/threonine-protein kinase that acts as a sensor of mitochondrial damage, phosphorylating proteins to coordinate mitochondrial quality control mechanisms that remove and replace dysfunctional components. Related references: PMID:40080546, PMID:14607334, PMID:15087508, PMID:18443288
• Upon irreversible mitochondrial stress, PINK1 accumulates at the outer mitochondrial membrane, phosphorylates polyubiquitin chains, recruits and activates PRKN, which ubiquitinates OMM proteins to initiate mitophagy. Related references: PMID:14607334, PMID:15087508, PMID:18443288, PMID:19966284
• The PINK1-PRKN pathway promotes mitochondrial fission by mediating phosphorylation and degradation of fusion proteins such as MFN2, preventing refusion and facilitating autophagic engulfment. Related references: PMID:18443288, PMID:23620051, PMID:24898855
• PINK1 also promotes fission independently of PRKN and ATG7-mediated mitophagy by phosphorylating and activating DNM1L. Related references: PMID:18443288, PMID:32484300
• It regulates damaged mitochondria motility by promoting ubiquitination and degradation of MIRO1 and MIRO2, likely inhibiting anterograde axonal transport to favor mitophagy in the soma. Related references: PMID:22396657
• Required for ubiquinone reduction by mitochondrial complex I through phosphorylation of complex I subunit NDUFA10 (By similarity).
• Phosphorylates LETM1, positively regulating its mitochondrial calcium transport activity. Related references: PMID:29123128
• Subcellular location: mitochondrion outer membrane, mitochondrion inner membrane, cytoplasm, cytosol. Highly expressed in heart, skeletal muscle, and testis; lower in brain, placenta, liver, kidney, pancreas, prostate, ovary, and small intestine. Also known as BRPK or PTEN-induced putative kinase protein 1; associated with Parkinson disease and neurodegeneration.

Experimental Guidance and Technical Tips

• Given the immunogen corresponding to amino acids 282–581, the antibody may recognize full-length PINK1 (~63 kDa) and potentially processed forms. For western blotting, validate using appropriate positive controls such as heart or skeletal muscle lysates and consider using mitochondrial enrichment.
• For immunofluorescence (IF/ICC), expect a mitochondrial staining pattern co-localizing with mitochondrial markers like Tom20; include mitophagy inducer (e.g., CCCP) treatments to observe accumulation.
• For IHC-P, test on known high-expression tissues (heart, skeletal muscle, testis) and perform antigen retrieval optimization. Cross-reactivity with mouse and rat allows for model organism studies.
• ELISA applications may be suitable for detecting PINK1 in cell or tissue lysates; however, pairing with validated standards is recommended.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a one-stop sourcing partner for IVD raw materials, enabling diagnostic developers to accelerate assay creation with high-quality antibodies and ancillary reagents. This anti-PINK1 polyclonal antibody supports research into mitochondrial dysfunction and Parkinson's disease biomarkers. From validated antibody pairs to bulk volume procurement, CamelBio streamlines your supply chain—helping you move from target discovery to clinical application efficiently.