Anti-PIN4 (Par14/Par17) Rabbit pAb for WB and ELISA - Q9Y237

Core Product Specifications and Parameters

Biological Background: PIN4 Function and Localization

• PIN4 encodes two isoforms, isoform 1 (Par14) and isoform 2 (Par17), produced by alternative promoter usage. Both belong to the parvulin family of peptidyl-prolyl cis-trans isomerases (PPIases) and function as rotamases.
• Isoform 1 (Par14) plays a role in ribosomal RNA processing and ribosome biogenesis. It also shows affinity for tightly bent AT-rich stretches of double-stranded DNA.
• Isoform 2 (Par17) is a DNA-binding protein that interacts with double-stranded DNA.
• The protein localizes to multiple subcellular compartments, including the nucleus, nucleolus, cytoplasm, cytoskeleton (spindle), and mitochondria (mitochondrial matrix).
• Post-translational modifications, such as phosphorylation and ubiquitin-like conjugation, have been identified, suggesting regulation of PIN4 activity.
• Expression is ubiquitous, with isoform 1 transcripts more abundant than isoform 2. Isoform 2 protein is more stable. Detected in tissues including kidney, liver, blood vessel, brain, mammary gland, skeletal muscle, small intestine, and submandibularis.

Experimental Guidance and Technical Tips

• For Western blotting, consider optimizing the antibody dilution as a polyclonal reagent. Validate the detection of both Par14 (~14 kDa) and Par17 (~17 kDa) isoforms, as the immunogen (aa 26-156) covers regions present in both.
• When using ELISA, a recombinant PIN4 protein containing the immunogen sequence may serve as an appropriate standard control. Confirm linearity and sensitivity in the relevant sample matrix.
• Cross-reactivity with mouse and rat is indicated; however, verify specificity and signal intensity in these species through pilot experiments. Endogenous PIN4 levels and isoform distribution may vary across tissues and cell lines.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with reliable access to IVD raw materials, including validated antibody pairs, monoclonal and polyclonal antibodies, and ancillary reagents. This PIN4 antibody supports investigations into ribosomal biogenesis and DNA-binding mechanisms. For bulk orders, customized formulations, or sourcing of rare-target reagents, contact our team to explore procurement solutions that streamline your workflow from concept to clinic.