Anti-Pin1 Polyclonal Antibody for WB, IF/ICC, ELISA - Q13526

Core Product Specifications and Parameters

Biological Background: Pin1 Function and Localization

• Pin1 (Peptidyl-prolyl cis-trans isomerase NIMA-interacting 1) is a peptidyl-prolyl cis/trans isomerase (PPIase) that specifically binds and isomerizes phosphorylated Ser/Thr-Pro motifs, acting as a molecular switch in multiple cellular processes. Related references: PMID:21497122 PMID:23623683 PMID:29686383
• Regulates mitosis by interacting with NIMA and attenuating its mitosis-promoting activity; also transactivates oncogenes and induces centrosome amplification, chromosome instability, and cell transformation. Related references: PMID:21497122 PMID:22033920
• Mediates phosphorylation-dependent protein degradation of targets such as PML, BCL6, FBXW7, and RBBP8/CtIP, influencing DNA repair pathway choice and oncogenic signaling. Related references: PMID:17828269 PMID:22608923 PMID:23623683 PMID:27561354
• Down-regulates kinase activity of BTK and is required for efficient dephosphorylation and recycling of RAF1 after mitogen activation. Related references: PMID:16644721 PMID:15664191
• Catalyzes isomerization of phosphorylated PGK1 to promote mitochondrial targeting under hypoxia, and of IRAK3 to induce pro-inflammatory gene expression in dendritic cells upon IL33 stimulation. Related references: PMID:26942675 PMID:29686383
• Localizes to the nucleus, nuclear speckles, and cytoplasm, reflecting its diverse functional roles.
• Tissue expression: detected in immune cells in the lung; phosphorylated form at Ser-71 is present in normal breast tissue cells but absent in breast cancer cells. Related references: PMID:29686383

Experimental Guidance and Technical Tips

• The immunogen covers the full-length human Pin1 protein (amino acids 1–163), encompassing both the WW domain and the PPIase catalytic domain. This design typically yields antibodies recognizing conserved epitopes, supporting cross-reactivity with mouse and rat.
• For Western blotting, consider optimizing primary antibody dilution based on endogenous Pin1 expression levels in your sample system. Positive controls can include HeLa, Jurkat, or A549 lysates.
• In IF/ICC, fix and permeabilize cells appropriately; expect nuclear and cytoplasmic staining patterns consistent with Pin1’s dual localization. Validate specificity using knockdown or knockout controls.
• For ELISA, the antibody can be used for capture or detection; pairing with a matched antibody or using recombinant Pin1 as standard is recommended.
• Always titrate the antibody in your specific assay conditions and include proper controls to ensure signal specificity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers and researchers a one‑stop sourcing solution for IVD raw materials, technical services, and consulting—from concept to clinic. For Pin1-focused projects in cell cycle and signal transduction, we provide reliable access to validated antibody pairs, optimized polyclonal and monoclonal antibodies, and bulk ancillary reagents. Whether you need rare target sourcing or end‑to‑end development support, CamelBio bridges your supply chain with quality and efficiency.