Anti-PIN1 Polyclonal Antibody for WB and ELISA - Q9C6B8

Core Product Specifications and Parameters

Biological Background: PIN1 Function and Localization

• PIN1 is an auxin efflux carrier component that transports auxins such as IAA, IBA, IPA, and 4-Cl-IAA; its activity is enhanced by D6PK-mediated phosphorylation. Related references: PMID:35917925
• It mediates basipetal auxin transport, establishing auxin gradients essential for proper organogenesis.
• The polar plasma membrane localization of PIN1 is regulated by vesicle trafficking and phosphorylation by PID kinase; GNOM (ARF-GEF) mediates its recycling between the membrane and endosomes.
• PIN1 recruits NPY proteins (e.g., NPY1/MAB4, NPY5/MEL1) to the plasma membrane at the basal side in root epidermis, a process optimized by AGC kinase phosphorylation to limit lateral diffusion. Related references: PMID:33705718
• Subcellular localization: Predominantly localized to the cell membrane.
• Tissue specificity: Expressed at the basal side of elongated parenchymatous xylem cells.
• Annotated with keywords including 3D-structure, auxin signaling pathway, glycoprotein, phosphoprotein, membrane, and transport.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the C-terminal region (aa 391–480) of Arabidopsis thaliana PIN1; consider this epitope location when assessing isoform cross-reactivity.
• For western blotting (WB), a band around 67 kDa is expected under denaturing conditions; validate in the relevant Arabidopsis thaliana sample system.
• For ELISA, consider coating with the recombinant immunogen or native protein extract for sensitivity optimization.
• As a polyclonal antibody, it may exhibit batch-to-batch variation; perform appropriate validation for each new lot.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio streamlines your IVD raw material procurement by offering validated antibody pairs, optimized polyclonal and monoclonal antibodies, and bulk ancillary reagents for auxin signaling and plant development research. For rare or custom targets like PIN1, we leverage a global network to secure high-quality materials with efficient lead times, supporting your transition from concept to clinic.