Anti-PIMREG Rabbit Polyclonal Antibody for WB, ELISA - Q9BSJ6

Core Product Specifications and Parameters

Biological Background: PIMREG Function and Localization

• Protein PIMREG (PICALM-interacting mitotic regulator), also known as FAM64A, CATS, or RCS1, is a mitotic regulator implicated in the control of metaphase-to-anaphase transition.
• Encoded by the PIMREG gene, alternative splicing generates multiple isoforms, and the protein features repeat regions and is subjected to phosphorylation and ubiquitin-like conjugation.
• Localizes primarily to the nucleus and nucleolus, consistent with its role in cell cycle progression and chromosome segregation.
• Functions during mitosis, likely through physical interactions with PICALM, to facilitate proper chromosome segregation.
• Expressed at the protein level in thymus, and detected in spleen, colon, ovary, and small intestine, indicating a role in proliferating tissues.
• Classified under keywords such as Cell cycle, Cell division, Mitosis, Nucleus, Phosphoprotein, and Ubl conjugation.

Experimental Guidance and Technical Tips

• The immunogen (recombinant fusion protein covering amino acids 1-100) is designed to target the N-terminal region; this region is conserved across isoforms, potentially enabling broad isoform detection.
• For Western blot, consider using lysates from thymus or spleen where PIMREG is expressed at the protein level, and validate with appropriate controls.
• Cross-reactivity with mouse and rat allows testing in rodent cell lines or tissues; confirm specificity by comparing with knockout or knockdown samples.
• In ELISA, the antibody may be used for capture or detection; optimize coating and detection conditions using recombinant PIMREG protein or cell lysates.
• Consider the 27 kDa predicted molecular weight, but note that post‑translational modifications or isoform variations might shift the observed band size in WB.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides reliable access to this PIMREG polyclonal antibody and a comprehensive portfolio of IVD raw materials, supporting research and diagnostic development from concept to clinic. Our offerings include validated antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk ancillary reagents, with dedicated sourcing support for rare targets in cell cycle and mitosis research. Partner with CamelBio to streamline your supply chain and accelerate your project timelines.