Anti-PIM3 Rabbit Polyclonal Antibody for WB and ELISA - Q86V86

Core Product Specifications and Parameters

Biological Background: PIM3 Function and Localization

• PIM3 is a serine/threonine-protein kinase and proto-oncogene belonging to the PIM kinase family. It functions as an ATP-binding kinase that phosphorylates target proteins to regulate cell survival and proliferation.
• The kinase prevents apoptosis and promotes protein translation, contributing to tumorigenesis through phosphorylation of BAD, which releases the anti-apoptotic protein Bcl-X(L), and by modulating MYC transcriptional activity.
• PIM3 also negatively regulates insulin secretion by inhibiting MAPK1/3 (ERK1/2) activation via SOCS6, and is involved in energy metabolism through regulation of AMPK activity, MYC, and PPARGC1A protein levels.
• Subcellular localization is primarily cytoplasmic, consistent with its role in signal transduction pathways.
• The protein is classified as a phosphoprotein and is subject to ubiquitin-like conjugation (Ubl), as indicated by proteomics identification and keyword annotations.
• Tissue-specific expression is detected in heart, brain, lung, kidney, spleen, placenta, skeletal muscle, and peripheral blood leukocytes, while expression is low or absent in normal adult endoderm-derived organs (colon, thymus, liver, small intestine) but becomes augmented in premalignant and malignant lesions.

Experimental Guidance and Technical Tips

• For Western blot, the predicted molecular weight is approximately 36 kDa. Optimize blocking and antibody dilution to minimize background, as polyclonal antibodies may recognize multiple epitopes.
• In ELISA, consider testing the antibody in both direct and capture formats, depending on assay design and required sensitivity.
• Since the immunogen corresponds to the C-terminal region (amino acids 247–326), the antibody is expected to detect full-length PIM3. Validate specificity in human samples, as cross-reactivity with other species has not been characterized.
• As a polyclonal antibody, it may provide robust signal across applications, but each new assay should be validated with appropriate positive and negative controls.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your reliable partner for IVD raw material sourcing, offering diagnostic manufacturers and research laboratories streamlined access to validated antibodies, bulk ancillary reagents, and rare target materials. This anti-PIM3 antibody supports oncology and kinase signaling research, and we can complement it with optimized monoclonal/polyclonal antibodies, recombinant proteins, and other custom IVD raw materials to accelerate your assay development from concept to clinic.