Anti-PIM2 Rabbit Polyclonal Antibody for WB, ELISA - Q9P1W9

Core Product Specifications and Parameters

Biological Background: PIM2 Function and Localization

• PIM2 (Pim-2h) is a serine/threonine-protein kinase and proto-oncogene that promotes cell survival and proliferation through phosphorylation of key substrates.
• It stabilizes the MYC transcription factor, enhancing its transcriptional activity, and regulates cap-dependent protein translation in an mTORC1-independent manner.
• PIM2 mediates anti-apoptotic signaling by phosphorylating BAD, which triggers release of Bcl-X(L), and activates the NF-kappa-B pathway via MAP3K8/COT phosphorylation.
• In cell cycle regulation, PIM2 phosphorylates the CDK inhibitors CDKN1A (p21) and CDKN1B (p27), fostering growth factor-independent proliferation.
• The kinase also contributes to chondrocyte survival and autophagy in the epiphyseal growth plate.
• It is highly expressed in hematopoietic tissues, various leukemic/lymphoma cell lines, testis, and colorectal adenocarcinoma, with elevated levels in hepatocellular carcinoma.
• Key structural features include ATP- and nucleotide-binding domains; it is a member of the protein kinase superfamily.

Experimental Guidance and Technical Tips

• For Western blot (WB), consider using whole-cell lysates from human or mouse hematopoietic cell lines (e.g., Jurkat, K562) or testis tissue, as PIM2 is highly expressed in these samples; the expected band size is ~34 kDa.
• For ELISA, the immunogen (a synthetic peptide within amino acids 100-200 of human PIM2) may serve as a positive control or coating antigen; validate assay conditions in the relevant sample matrix.
• When probing mouse samples, confirm sequence homology in the targeted region (aa 100-200) as cross-reactivity is reported but may require verification.

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CamelBio is your one-stop sourcing partner for IVD raw materials, offering validated antibody pairs, optimized polyclonal antibodies like this anti-PIM2 antibody, and bulk ancillary reagents to support your oncology research and diagnostic development. From concept to clinic, our technical services and consultation help streamline your procurement of rare targets and essential assay components.