Anti-PIM2 Rabbit Monoclonal Antibody for WB, IP, ELISA - Q9P1W9

Core Product Specifications and Parameters

Biological Background: PIM2 Function and Localization

• PIM2 encodes a serine/threonine-protein kinase that functions as a proto-oncogene, promoting cell survival and proliferation through multiple signaling pathways.
• It regulates MYC transcriptional activity by phosphorylating MYC, increasing its protein stability and transcriptional output, contributing to tumorigenesis.
• PIM2 mediates survival signaling by phosphorylating the pro-apoptotic protein BAD, leading to release of the anti-apoptotic protein Bcl-X(L).
• The kinase promotes cap-dependent protein translation in an mTORC1-independent manner, acting in parallel to the PI3K-Akt pathway.
• It positively regulates the I-kappa-B kinase/NF-kappa-B cascade through phosphorylation of MAP3K8/COT, enhancing cell survival.
• PIM2 is highly expressed in hematopoietic tissues and various cancer cell lines, with elevated levels observed in hepatocellular carcinoma compared to normal liver.
• Keywords associated with PIM2 include ATP-binding, kinase, proto-oncogene, and involvement in cell cycle and apoptosis.

Experimental Guidance and Technical Tips

• The immunogen corresponds to a sequence within amino acids 200-300 of human PIM2; the antibody may recognize endogenous PIM2 in relevant samples.
• For Western blot, a band around 34 kDa is expected based on the predicted molecular weight; consider using appropriate loading controls and verification with positive cell lines.
• For immunoprecipitation, validate the antibody's ability to pull down PIM2 in the relevant cell system, and optimize lysis buffer conditions.
• ELISA applications may require titration of the antibody; ensure the coating antigen is compatible with the peptide immunogen.
• Cross-reactivity is listed as human; if using other species, verify reactivity experimentally.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials. For PIM2-related research, we offer validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents tailored to your assay development needs. Our team also assists in sourcing rare target raw materials, supporting your projects from concept to clinic.