Anti-PIM2 Rabbit Monoclonal Antibody for WB, ELISA - Q9P1W9

Core Product Specifications and Parameters

Biological Background: PIM2 Function and Localization

• Serine/threonine-protein kinase pim-2 (Pim-2h) is a proto-oncogene that plays critical roles in cell survival and proliferation, primarily through the regulation of key signaling pathways.
• PIM2 enhances MYC transcriptional activity by phosphorylating MYC, which increases MYC protein stability and potentiates its oncogenic effects, explaining the synergism between PIM2 and MYC in tumorigenesis.
• It regulates cap-dependent protein translation independently of mTORC1 and in parallel to the PI3K-Akt pathway, thereby controlling protein synthesis in response to growth signals.
• PIM2 promotes cell survival by phosphorylating the pro-apoptotic protein BAD, leading to the release of the anti-apoptotic protein Bcl-X(L)/BCL2L1 and activation of the I-kappa-B kinase/NF-kappa-B cascade via MAP3K8/COT phosphorylation.
• The kinase also drives cell cycle progression by phosphorylating cell cycle inhibitors such as CDKN1A (p21) and CDKN1B (p27), enabling growth factor-independent proliferation.
• Highly expressed in hematopoietic tissues, leukemic and lymphoma cell lines, testis, small intestine, colon, and colorectal adenocarcinoma cells, with low expression in normal liver but elevated in hepatocellular carcinoma, highlighting its role in blood and solid tumors.
• Belongs to the protein kinase superfamily and contains an ATP-binding site; classified under keywords including apoptosis, cell cycle, nucleotide-binding, and proto-oncogene.

Experimental Guidance and Technical Tips

• The immunogen used for this rabbit monoclonal antibody corresponds to amino acids 10–120 of human PIM2, targeting the N-terminal region, which is present in all known isoforms; ensure this region is retained in your target sample.
• For Western blot (WB), a band around 34 kDa is expected (full-length PIM2). Optimize antibody dilution (e.g., 1:500–1:2000) based on sample type and detection sensitivity; use validated positive controls (e.g., HEK293, HeLa, or lymphoid cell lines).
• In ELISA applications, this monoclonal antibody can be used as a capture or detection reagent; pairing with a compatible partner antibody is recommended for sandwich assays. Cross-reactivity with human, mouse, and rat PIM2 makes it suitable for multi-species studies.
• Although not tested in other applications, consider validating in IP, IHC, or flow cytometry if needed, as monoclonal antibodies often show versatility; preliminary testing with appropriate controls is advised.
• Store and handle according to the shipping condition (ice bag) and avoid repeated freeze-thaw cycles to maintain antibody integrity; aliquot upon receipt if frequent use is anticipated.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio simplifies IVD raw material procurement by offering a comprehensive portfolio that includes validated antibody pairs, monoclonal/polyclonal antibodies, bulk ancillary reagents, and specialized sourcing for rare targets. This PIM2 Rabbit mAb serves as a reliable tool for exploring oncogenic signaling, cell survival mechanisms, and cancer biomarker development. Whether you need scale-up quantities, technical consultation, or custom assay support, CamelBio bridges the gap from concept to clinic with seamless supply chain solutions.