Anti-PIM1 Rabbit Monoclonal Antibody for WB, IHC-P, ELISA - P11309

Core Product Specifications and Parameters

Biological Background: PIM1 Function and Localization

• PIM1 is a serine/threonine-protein kinase that acts as a proto-oncogene, promoting cell survival and proliferation and providing a selective advantage in tumorigenesis. Related references: PMID:15528381, PMID:1825810, PMID:31548394
• It exerts oncogenic activity by regulating MYC transcriptional activity and phosphorylating/inhibiting proapoptotic proteins BAD, MAP3K5, and FOXO3. Related references: PMID:18593906
• PIM1 phosphorylates MAP3K5, reducing its kinase activity and inhibiting downstream JNK/p38MAPK-mediated caspase-3 activation and apoptosis. Related references: PMID:19749799
• The kinase promotes cell cycle progression at G1-S and G2-M transitions by phosphorylating CDC25A and CDC25C, and regulates CDKN1A and CDKN1B stability and localization. Related references: PMID:16356754, PMID:12431783, PMID:18593906
• PIM1 may influence chromatin structure through phosphorylation of HP1 gamma/CBX3 and positively regulates mTORC1 signaling by phosphorylating and inhibiting DEPDC5. Related references: PMID:10664448, PMID:31548394
• It phosphorylates and activates ABCG2, contributing to drug resistance, and acts as a negative regulator of innate immunity by phosphorylating GBP1. Related references: PMID:18056989, PMID:37797010
• PIM1 localizes to the cytoplasm, nucleus, and cell membrane. It is predominantly expressed in hematopoietic and germline tissues, with isoforms detected in prostate cancer.

Experimental Guidance and Technical Tips

• The immunogen used is a synthetic peptide corresponding to the N-terminal region (aa 1-100) of human PIM1. This region is conserved across human, mouse, and rat, contributing to cross-reactivity. Verify sequence homologies for other species if needed.
• For Western blotting (WB), a specific band at approximately 36 kDa is expected. Include appropriate positive (e.g., HeLa, Jurkat) and negative controls to confirm specificity.
• For immunohistochemistry (IHC-P), standard antigen retrieval methods may be required. Consider testing multiple retrieval buffers and optimize antibody dilution based on tissue type.
• In ELISA, use recombinant or purified PIM1 protein as the coating antigen. Perform checkerboard titrations to determine optimal antibody and antigen concentrations.
• Due to the protein’s involvement in multiple signaling pathways, results should be validated using complementary approaches such as siRNA-mediated knockdown or overexpression assays.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio specializes in providing one-stop access to high-quality IVD raw materials, including validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and sourcing for rare targets. For PIM1-related research and diagnostic applications in oncology, our portfolio supports the development of robust immunoassays. Beyond this product, CamelBio offers comprehensive technical consultation and supply chain solutions to help diagnostic manufacturers and laboratories accelerate their programs from concept to clinic.