Anti-PIKFYVE Rabbit Polyclonal Antibody for WB, ELISA - Q9Y2I7

Core Product Specifications and Parameters

Biological Background: PIKFYVE Function and Localization

• PIKFYVE, also known as PIP5K3, is a dual-specificity lipid and serine-protein kinase that serves as the sole enzyme responsible for synthesizing phosphatidylinositol 3,5-bisphosphate (PtdIns(3,5)P2) from phosphatidylinositol 3-phosphate (PubMed:17556371, PubMed:41639454). Related references: PMID:17556371 | PMID:41639454
• The protein contains an N-terminal FYVE zinc finger domain that targets it to endosomal membranes, where it primarily localizes to early and late endosomes, phagosomes, and cytoplasmic vesicles.
• PIKFYVE is a critical regulator of endomembrane homeostasis, controlling endosome fission, fusion, and maturation; it is essential for the transition from early to late endosomes and for lysosomal function (PubMed:30612035, PubMed:22621786). Related references: PMID:30612035 | PMID:22621786
• Through its lipid kinase activity, PIKFYVE regulates autophagy, vacuole maturation, and nutrient recovery by facilitating the recycling of lysosomal contents back into the endosome network (PubMed:27623384). Related references: PMID:27623384
• The kinase also plays a role in insulin signaling and GLUT4 translocation, thereby contributing to systemic glucose homeostasis (By similarity).
• In immune cells, PIKFYVE is required for phagosome–lysosome fusion, ROS generation, and antigen presentation via MHC class II, processes vital for innate and adaptive immunity (PubMed:28779020, PubMed:30612035). Related references: PMID:28779020 | PMID:30612035
• PIKFYVE autophosphorylation downregulates its lipid kinase activity and stimulates the lipid phosphatase FIG4, creating a regulatory loop that fine-tunes PtdIns(3,5)P2 levels (PubMed:33098764). Related references: PMID:33098764
• Notably, the enzyme is hijacked by coronaviruses such as SARS-CoV and SARS-CoV-2 to facilitate cell entry via endocytosis.

Experimental Guidance and Technical Tips

• Given the immunogen spans amino acids 269–545 of human PIKFYVE, the antibody is expected to recognize the endogenous protein across human, mouse, and rat samples. For Western blotting, note the large molecular weight (~237 kDa); use a low-percentage acrylamide gel (e.g., 6–8%) and extended transfer times to ensure efficient protein transfer.
• For ELISA, this antibody can be used as a capture or detection reagent for recombinant PIKFYVE protein; confirm optimal coating concentrations empirically.
• Consider including appropriate positive and negative controls, such as lysates from cells with known PIKFYVE expression, and validate the antibody in the relevant experimental system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio partners with diagnostic manufacturers, research laboratories, and academic institutes to provide high-quality IVD raw materials and technical support. This anti-PIKFYVE polyclonal antibody is sourced for studies of endolysosomal trafficking, metabolic regulation, and viral entry mechanisms. Beyond single antibodies, CamelBio offers validated antibody pairs, bulk lot orders, and customized procurement solutions to accelerate your assay development and research workflows.