Anti-PIK3R4/VPS15 Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - Q99570

Core Product Specifications and Parameters

Biological Background: PIK3R4/VPS15 Function and Localization

• PIK3R4/VPS15, also known as PI3-kinase p150 subunit or phosphoinositide 3-kinase adaptor protein, functions as a regulatory subunit of the class III PI3K complex, mediating synthesis of phosphatidylinositol 3-phosphate (PI3P) on endosomal and autophagosomal membranes.
• The protein is a key component of two main PI3KC3 subcomplexes: PI3KC3-C1, which initiates autophagosome formation, and PI3KC3-C2, which facilitates autophagosome maturation and endocytosis.
• PIK3R4/VPS15 is involved in the regulation of degradative endocytic trafficking and cytokinesis, likely via PI3KC3-C2 complex activity. Related references: PMID:20643123
• It localizes primarily to late endosomes, autophagosomes, cytoplasmic vesicles, and membranes, consistent with its trafficking roles.
• The protein contains a serine/threonine kinase domain and multiple WD40 repeats, and is subject to post-translational modifications including myristoylation and phosphorylation (UniProt keyword: Myristate, Phosphoprotein).
• Ubiquitously expressed across human tissues, PIK3R4/VPS15 is essential for basal macroautophagy and intracellular sorting.

Experimental Guidance and Technical Tips

• The immunogen used corresponds to the N-terminal 1-330 amino acids of human PIK3R4/VPS15, which may detect full-length protein as well as potential processing products or degradation fragments. Validate specificity in your experimental system.
• For Western blotting, note the predicted molecular weight of ~153 kDa; consider using a 6-8% SDS-PAGE gel and extended transfer times to ensure efficient transfer of this high-molecular-weight protein.
• In IHC-P and IF/ICC applications, expect punctate cytoplasmic staining corresponding to late endosomes and autophagosomes; include appropriate controls to confirm subcellular localization.

CamelBio: Your One-Stop Sourcing Bridge

Diagnostic manufacturers and research laboratories trust CamelBio as a single-source partner for high-quality IVD raw materials, from target discovery to clinical application. For investigations into autophagy, PI3K signaling, or membrane trafficking, we offer pre-validated antibody pairs, polyclonal and monoclonal antibodies, bulk ancillary reagents, and sourcing assistance for rare targets. Let CamelBio streamline your supply chain so you can focus on assay development with confidence.