Anti-PIK3R4/VPS15 Monoclonal Antibody for WB, IF/ICC, ELISA - Q99570

Core Product Specifications and Parameters

Biological Background: PIK3R4/VPS15 Function and Localization

• PIK3R4, also known as VPS15 or p150, is the regulatory subunit of the class III phosphatidylinositol 3-kinase (PI3KC3) complex that produces phosphatidylinositol 3-phosphate (PI3P) essential for membrane trafficking.
• As part of the PI3KC3-C1 complex, PIK3R4/VPS15 is involved in autophagosome initiation; within the PI3KC3-C2 complex, it contributes to autophagosome maturation and endocytic trafficking. Related references: PMID:20643123
• The protein contains WD repeats, a serine/threonine kinase domain, and an N-terminal myristate anchor, highlighting its regulatory kinase and scaffolding roles.
• PIK3R4 localizes to late endosomes, autophagosomes, and cytoplasmic vesicles, consistent with its role in endosomal and autophagy pathways.
• It is ubiquitously expressed across human tissues, indicating a fundamental housekeeping function in membrane dynamics.
• Alternative names include PI3-kinase p150 subunit and phosphoinositide 3-kinase adaptor protein.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 1259-1358 of human PIK3R4; consider epitope accessibility in native protein detection assays.
• For Western blot (WB), the predicted molecular weight is ~153 kDa; optimize gel percentage and transfer conditions for high-molecular-weight proteins.
• For immunofluorescence (IF/ICC), validate fixation and permeabilization protocols to preserve organelle morphology, given the protein's association with endosomes and autophagosomes.
• When using ELISA, pair with appropriate capture/detection antibodies and validate in the relevant sample system.
• Cross-reactivity with mouse has been reported; verify reactivity in other species if needed.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers and research labs with one-stop access to IVD raw materials and technical services. This PIK3R4/VPS15 antibody serves targets in autophagy, endocytosis, and kinase signaling—areas where CamelBio offers validated antibody pairs, optimized monoclonal antibodies, and ancillary reagents. For rare-target sourcing or bulk supply, CamelBio's consulting and procurement capabilities streamline your workflow from concept to clinic.