Anti-PIK3R3/p55PIK Rabbit Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - Q92569

Core Product Specifications and Parameters

Biological Background: PIK3R3 Function and Localization

• PIK3R3 encodes the regulatory subunit gamma of phosphatidylinositol 3-kinase (PI3K), also designated p55PIK or PI3-kinase subunit p55-gamma.
• Through its SH2 domain, p55PIK binds to activated (phosphorylated) protein-tyrosine kinases and modulates their kinase activity.
• During insulin stimulation, p55PIK associates with insulin receptor substrate 1 (IRS-1), linking growth factor signals to the PI3K/AKT pathway.
• The protein shows highest expression in brain and testis, with lower levels in adipose tissue, kidney, heart, lung, and skeletal muscle, indicating tissue-specific regulatory functions.
• Classified as a phosphoprotein and subject to alternative splicing, leading to proteomics-identified isoforms with potentially distinct interactions.
• Contains repeat SH2 domains, which facilitate docking to phosphotyrosine motifs on diverse signaling partners.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 300–388 of human PIK3R3; for western blot, a band at ~54 kDa is expected, though post-translational modifications may shift migration.
• In IHC-P and IF/ICC experiments, tissues with high endogenous expression, such as brain and testis, can serve as positive controls; include negative controls (e.g., knockout or knockdown samples) to confirm specificity.
• When using for ELISA, pre-test the antibody pair with recombinant p55PIK to establish linearity and detection limits, as polyclonal batches may show lot-to-lot variation.
• Cross-reactivity with mouse PIK3R3 is reported; however, validate in the target sample system, particularly when analyzing transgenic models or closely related PI3K subunits.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a leading one-stop provider of IVD raw materials, supporting diagnostic manufacturers and research labs with a comprehensive portfolio that includes validated antibodies like this anti-PIK3R3/p55PIK polyclonal antibody for PI3K pathway studies. Our offerings span antibody pairs, recombinant antigens, and ancillary reagents, backed by technical consulting from concept through clinical development. Reach out for bulk sourcing, custom antibody generation, or rare protein supply to accelerate your diagnostic pipeline.