Anti-PIK3R3/p55PIK Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - Q92569

Core Product Specifications and Parameters

Biological Background: PIK3R3/p55PIK Function and Localization

• PIK3R3 encodes the 55 kDa regulatory subunit gamma (p55PIK) of phosphatidylinositol 3-kinase (PI3K), a lipid kinase critical for signal transduction.
• The p55PIK protein contains an SH2 domain that binds to phosphorylated tyrosine residues on activated protein-tyrosine kinases, thereby regulating their kinase activity.
• During insulin stimulation, p55PIK binds to insulin receptor substrate 1 (IRS-1), playing a role in insulin signaling and metabolic regulation.
• Tissue expression is highest in brain and testis, with lower levels detected in adipose tissue, kidney, heart, lung, and skeletal muscle.
• The protein undergoes alternative splicing and is a phosphoprotein, indicating potential regulation by post-translational modifications.

Experimental Guidance and Technical Tips

• The immunogen corresponds to an internal sequence (aa 300–388) of human p55PIK. This design may target a region conserved across human, mouse, and rat, supporting the observed cross-reactivity.
• For Western blot (WB), consider loading 20–30 µg of whole-cell lysate and using a dilution optimized for the predicted 54 kDa band.
• In immunofluorescence (IF/ICC) and ELISA, pre-test the antibody concentration in the relevant cellular or tissue system to confirm specificity, especially given the high expression in brain and testis.
• Cross-reactivity with mouse and rat has been documented, but validation in each species’ sample type is recommended.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers, labs, and research institutes streamlined access to premium IVD raw materials. This PIK3R3/p55PIK antibody supports investigations into PI3K signaling, insulin pathways, and related disease research. Beyond individual reagents, CamelBio supplies validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and sourcing for rare-target raw materials to accelerate your concept-to-clinic journey.