Anti-PIK3R3/p55PIK Monoclonal Antibody for WB - Q92569

Core Product Specifications and Parameters

Biological Background: PIK3R3/p55PIK Function and Localization

• PIK3R3 encodes the phosphatidylinositol 3-kinase regulatory subunit gamma, also known as p55PIK or p55-GAMMA.
• The protein contains an SH2 domain, enabling binding to activated (phosphorylated) protein-tyrosine kinases.
• During insulin stimulation, p55PIK binds to insulin receptor substrate 1 (IRS-1), regulating downstream kinase activity.
• Predicted molecular weight is approximately 54 kDa.
• Tissue expression is highest in brain and testis, with lower levels in adipose tissue, kidney, heart, lung, and skeletal muscle.
• Classified as a phosphoprotein and subject to alternative splicing.
• Relevant keywords: Alternative splicing, Phosphoprotein, SH2 domain, Reference proteome.

Experimental Guidance and Technical Tips

• The immunogen corresponds to a synthetic peptide within amino acids 362-461 of human PIK3R3, which may recognize multiple isoforms or splice variants.
• For Western blot, consider using brain or testis tissue lysates as positive controls given the high expression levels in these tissues.
• Validate the antibody in your specific sample system and optimize dilutions for ELISA or WB applications.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio connects diagnostic manufacturers and research institutions with a comprehensive range of IVD raw materials. For studies on PI3K signaling and insulin pathways, we offer validated antibody pairs, optimized monoclonal antibodies like this anti-PIK3R3/p55PIK clone, and ancillary reagents to support your assay development and scale-up needs. Let us streamline your raw material sourcing from concept to clinic.