Anti-PIK3IP1 Rabbit Polyclonal Antibody for WB, ELISA - Q96FE7

Core Product Specifications and Parameters

Biological Background: PIK3IP1 Function and Localization

• PIK3IP1, also known as HGFL or TrIP, is a kringle domain-containing protein that acts as a negative regulator of hepatic phosphatidylinositol 3-kinase (PI3K) signaling.
• The protein is encoded by the PIK3IP1 gene (GeneID: 113791) and undergoes alternative splicing, yielding multiple isoforms.
• PIK3IP1 localizes to the cell membrane, consistent with its transmembrane helix and signal peptide.
• It contains a kringle domain, a conserved protein fold involved in protein-protein interactions, and is predicted to be a glycoprotein with disulfide bonds.
• UniProt keyword annotations indicate that PIK3IP1 is a membrane-associated, proteolytically processed protein with a molecular weight of approximately 28 kDa (UniProt: 28248 Da).

Experimental Guidance and Technical Tips

• Given the immunogen spans the extracellular region (aa 24-160), the antibody likely recognizes native and denatured PIK3IP1, making it suitable for applications like Western blot (WB) and ELISA. For WB, a band around 28 kDa is expected; consider using liver-derived cell lines as positive controls, given the hepatic role of PIK3IP1.
• Because this is a polyclonal antibody, lot-to-lot variation may occur; include appropriate controls and validate in your specific sample system.
• For ELISA, test both direct and capture formats to determine optimal sensitivity and specificity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supplies high-quality IVD raw materials, including validated antibody pairs and bulk reagents, to support diagnostic development. This anti-PIK3IP1 polyclonal antibody is part of our extensive portfolio for PI3K pathway research and assay design. For custom sourcing of rare targets or ancillary reagents, CamelBio offers tailored consulting and one-stop procurement solutions.