Anti-PIK3CG Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - P48736

Core Product Specifications and Parameters

Biological Background: PIK3CG Function and Localization

• PIK3CG encodes the catalytic subunit gamma isoform of phosphoinositide-3-kinase (PI3K), also known as p110gamma or p120-PI3K. It is a dual-specificity kinase with both lipid and serine/threonine protein kinase activities.
• The kinase phosphorylates PtdIns(4,5)P2 to generate PIP3, a critical second messenger that recruits PH domain-containing proteins like AKT1 and PDPK1 to the membrane, activating downstream signaling cascades involved in cell growth, survival, proliferation, motility, and morphology.
• PIK3CG links G protein-coupled receptor (GPCR) activation to PIP3 production, playing a central role in immune, inflammatory, and allergic responses. It modulates leukocyte chemotaxis, dendritic cell motility, T-lymphocyte migration and proliferation, B-lymphocyte development, and natural killer cell function.
• The protein is localized in the cytoplasm and at the cell membrane, where it participates in dynamic signaling assemblies.
• PIK3CG is highly expressed in pancreas, skeletal muscle, liver, and heart. In cardiac tissue, it acts as a negative regulator of contractility and is involved in cardiac hypertrophy under pathological stress.
• The enzyme also functions in platelet aggregation and thrombosis, endothelial progenitor cell migration, and angiogenesis, often through interactions with other PI3K subunits or signaling partners such as RASGRP4 and GRK2.
• Post-translational modifications include phosphorylation, and the protein contains ATP-binding and nucleotide-binding domains. It is classified as a transferase and a serine/threonine-protein kinase.
• Disease-associated variants of PIK3CG are linked to immune deficiencies and inflammatory disorders, underscoring its importance in human health.

Experimental Guidance and Technical Tips

• The immunogen corresponds to a region within the N-terminal half of PIK3CG (aa 130-342), which may confer reactivity across human, mouse, and rat due to high sequence conservation in this domain.
• For western blot (WB), note the predicted molecular weight is 126 kDa; use appropriate gel percentage and transfer conditions for high molecular weight proteins.
• In immunohistochemistry (IHC-P) and immunofluorescence (IF/ICC), verify antibody specificity using appropriate controls such as peptide blocking or siRNA knockdown, as polyclonal antibodies may exhibit background staining.
• Consider ELISA applications for quantitative detection, but ensure the antibody pair does not compete with the immunogen if used in sandwich formats.
• Cross-reactivity with mouse and rat samples is expected; however, validation in the actual sample system is recommended.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio streamlines the procurement of critical raw materials for diagnostic development, offering a comprehensive portfolio of antibodies, antigens, and ancillary reagents. This anti-PIK3CG antibody supports research into PI3K signaling, immune cell function, and cardiac physiology. Our global sourcing network ensures rapid access to validated antibody pairs, bulk reagents, and custom development services to accelerate your IVD project from concept to clinic.