Anti-PIK3CG Polyclonal Antibody for IHC-P, ELISA - P48736

Core Product Specifications and Parameters

Biological Background: PIK3CG Function and Localization

• PIK3CG encodes the catalytic subunit p110γ of class I phosphoinositide 3-kinase (PI3K) gamma, also known as PI3Kgamma or p120-PI3K, and belongs to the PI3K family of lipid kinases that phosphorylate phosphoinositides.
• The enzyme specifically phosphorylates phosphatidylinositol 4,5-bisphosphate (PtdIns(4,5)P2) to generate phosphatidylinositol 3,4,5-trisphosphate (PIP3), a critical second messenger that recruits PH domain-containing proteins like AKT1 and PDPK1 to the plasma membrane to activate downstream signaling cascades.
• PIK3CG links G protein-coupled receptor (GPCR) activation to PIP3 production and plays essential roles in immune, inflammatory, and allergic responses by modulating leukocyte chemotaxis, polarization, and migration.
• The protein is crucial for the development and function of various immune cells, including T lymphocytes (proliferation, activation, cytokine production), B lymphocytes, natural killer (NK) cells, dendritic cells, and neutrophils (respiratory burst, chemotaxis, extravasation).
• Together with PIK3CB, PIK3CG promotes platelet aggregation and thrombosis, and regulates alpha-IIb/beta-3 integrin adhesive function in platelets downstream of P2Y12.
• Beyond its lipid kinase activity, PIK3CG also possesses serine/threonine protein kinase activity, and both activities are required for beta-adrenergic receptor endocytosis; it also acts as a scaffold in modulating cardiac contractility and contributes to cardiac hypertrophy under pathological stress.
• Subcellularly, PIK3CG localizes primarily to the cytoplasm and the cell membrane, where it catalyzes PIP3 formation.
• Tissue expression is highest in the pancreas, skeletal muscle, liver, and heart; genetic variants are associated with immunodeficiency (IMD97).

Experimental Guidance and Technical Tips

• The immunogen, spanning amino acids 201-330 of human PIK3CG, targets a region outside the catalytic domain, which may help reduce cross-reactivity with other PI3K isoforms; validate in the relevant species and tissue context.
• For IHC-P, consider optimizing antigen retrieval methods, as the protein may be membrane- or cytoplasmic-localized; use known positive controls such as human pancreas or immune tissue sections.
• For ELISA, the antibody may be used to detect recombinant or native PIK3CG; establish standard curves with the immunogen or full-length protein.
• Due to the polyclonal nature, lot-to-lot variability is possible; users should validate each new lot in their specific assay.
• The antibody is raised in rabbit and reacts with human protein; cross-reactivity with other species has not been tested, so caution is advised when applying to non-human samples.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers and research institutions worldwide by providing high-quality raw materials like this anti-PIK3CG polyclonal antibody for immune signaling and inflammation research. We offer one-stop access to validated antibodies, bulk reagents, and customized sourcing for rare targets, streamlining your assay development from concept to clinic. Whether you need matched antibody pairs, antigen-grade proteins, or technical consultation, our integrated supply chain ensures consistent quality and timely delivery.