Anti-PIK3C2A Rabbit Polyclonal Antibody for WB, IHC-P, IF/ICC, IP, ELISA - O00443

Core Product Specifications and Parameters

Biological Background: PIK3C2A Function and Localization

• PIK3C2A encodes the catalytic subunit of class II phosphoinositide 3-kinase C2 alpha (PI3K-C2α), a lipid kinase that generates the second messengers phosphatidylinositol 3-phosphate (PtdIns3P) and phosphatidylinositol 3,4-bisphosphate (PtdIns(3,4)P2).
• Plays a central role in intracellular trafficking, including clathrin-coated endocytic vesicle formation, dynamin-independent endocytosis, and trafficking of ciliary components.
• Mediates insulin signaling and glucose homeostasis: required for GLUT4 translocation to the plasma membrane and glucose uptake, and participates in glucose-induced insulin secretion via AKT1 activation and insulin granule exocytosis.
• Subcellular localization includes the cell membrane, clathrin-coated vesicles, cytoplasm, nucleus, Golgi apparatus, and trans-Golgi network, reflecting its dynamic trafficking functions.
• Involved in the regulation of ciliogenesis and trafficking of ciliary components. Related references: PMID:31034465
• Expressed in a variety of tissues, with highest levels in heart, placenta, and ovary; also detected in endothelial cells, smooth muscle, and pancreatic islets, with reduced expression in type 2 diabetic islets.
• Post-translational modifications include acetylation and phosphorylation; keywords also highlight disease associations (cataract, dwarfism) though mechanistic details are limited.
• Alternative splicing yields multiple isoforms, potentially with distinct functional roles.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal region (aa 1–200) of human PIK3C2A; it may detect all known isoforms, but verification with isoform-specific controls is advised.
• Predicted molecular weight is ~191 kDa; for western blotting, use a 6–8% SDS-PAGE gel and optimize transfer conditions for high-molecular-weight proteins.
• Validated applications (WB, IHC-P, IF/ICC, IP, ELISA) are for human, mouse, and rat samples; each assay may require individual optimization of antibody dilutions and detection protocols.
• For immunohistochemistry on paraffin sections, antigen retrieval (heat-induced or enzymatic) may be necessary; consider testing both methods.
• In immunofluorescence, colocalization with organelle markers (e.g., TGN46, clathrin) can confirm expected subcellular distribution.
• Insulin stimulation or related treatments may reveal dynamic translocation responses in appropriate cell models, given PIK3C2A’s role in insulin signaling.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a comprehensive portfolio of IVD raw materials, including validated antibody pairs, recombinant proteins, and ancillary reagents for developing diagnostic assays targeting PI3K signaling and intracellular trafficking pathways. Our one-stop sourcing platform streamlines procurement from research to clinical application, with technical consultation and custom reagent development to accelerate your diagnostic pipeline.